Effect of Salinity and Drought Stress on Germination Characteristics and Seedling Growth of Sophora (Sophora pachycarpa Schrenk ex C. A. Mey)

Document Type : Research Article

Authors

• Fariba Pourhassan
• Ebrahim Izadi Darbandi
• Mohammad Hassan Rashed Mohassel

Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

10.22067/jpp.2024.80242.1120

Abstract

Introduction
Sophora (Sophora pachycarpa Schrenk ex C. A. Mey) is a perennial weed of Fabaceae family which can propagated by seeds and through perennial roots. It is one of the invasive plants that can be seen in the most regions of Iran, especially in the wheat fields, pastures and drylands, of Khorasan. The biology of weeds and its importance in management has received a lot of attention in recent years. In fact, it is necessary to know the biological characteristics and key relationships between weeds and crops to control weeds in an agricultural ecosystem. Also, the role of environmental conditions on weed management is very important. Given this, the present study seeks to investigate the effects of environmental conditions (salinity and drought stress) on germination characteristics of Sophora.
 
Materials and Methods
In order to investigate the effect of salinity and drought stress on germination characteristics and seedling growth of Sophora, two separate experiments were conducted in germination stage as a completely randomized design with four replications in the year 2017-2018 in research laboratories at Agricultural College of Ferdowsi University of Mashhad. PEG 6000 was used for drought stress and NaCl, for salinity stress. The treatments included drought and salinity stress levels (0, -0.1, -0.2, -0.4, -0.8, -1, -1.2, -1.4 and -1.5 Mpa). Seeds were disinfected with sodium hypochlorite (1%) solution for 2 minutes, and were then washed with distilled water. The measured traits were germination percentage, germination rate, average germination time, radicle length, plumule length and fresh weight of radicle, plumule and seedling. The statistical analysis of the data was done by SAS 9.1 and Sigma Plot 12.3 software.
 
Results and Discussion
Analysis of variance results showed that the effect of salinity levels on all traits was very significant (P≤ 0.01). Maximum germination was observed in control treatment and at the level of -1.5 Mpa salinity, the germination stopped. Also, the effect of drought levels on all traits was very significant (P≤ 0.01) and at the level of -1 Mpa drought, the germination stopped. Fitting of the three-parameter sigmoid model provided a successful estimation of the relationship between salt and drought stress levels and germination percentage of Sophora.This model showed that salinity and drought stress at -1.08 and -0.43 Mpa, respectively, caused a 50% reduction in maximum germination percentage of sophora, and it can be concluded that the sensitivity of this weed to drought is more than to salinity, and the existence of salinity and drought stress can decrease growth indices of this weed at germination and seedling stages.
Conclusions
It was concluded that with the increase in both the drought and salinity stress, all the growth characteristics of Sophora significantly decreased and the effect of salinity stress on percentage of germination and germination rate resulted from the toxic effect of ion salts. Whereas, the reduction of seedling growth traits was both the toxic effect of ion salts and osmotic potential. It seems that sufficient information about this weed is vital for the selection of the best control method, could help us to come up with new control approaches for this invasive weed. Considering the increase of salinity and drought in the country's soils due to the decrease of rainfall and climate change, and with the according to Sophora tolerance to salinity and its relative tolerance to drought, there is a possibility of expanding the range of presence of this weed in the conditions of salinity and drought of the soil, but for more accurate evaluation, it is necessary to conduct complementary experiments in the field and in the greenhouse conditions.

Keywords

• Germination percentage
• Germination rate
• Polyethylene glycol
• Sodium chloride

Main Subjects

• Weed

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©2024 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

https://doi.org/10.22067/JPP.2024.80242.1120

References

1. Ahmadvand, G., Dehghan Banadaki, M., Alimoradi, J., Goudarzi, S., & Ardalani, S. (2018). Reaction of germination and seedling growth of redroot pigweed (Amaranthus retroflexus) to salinity and drought stress. Iranian Journal of Seed Research, 4(2), 23-35. (In Persian with English abstract). http://doi.org/10.29252/yujs.4.2.23  
2. Alebrahim, M.T., Sharifi, K., & Darwishi, M. (2013). Effects of salinity stress on Prosopis farcta seed germination. Iranian Weed Science Conference. (In Persian with English abstract)
3. Amiri, B., Rasouli, B., Assareh, M.H., Jafari, M., & Jafari, A.A. (2012). Effect of NaCl & Na₂SO₄ on germination and seedling growth of Salicornia herbacea & Alhagi persarum. Iranian Journal of Range and Desert Research, 19(2), 233-243.‏ (In Persian with English abstract)
4. Ansari, , Salehi, A., Dehnavi, M.M., & Heydari, S. (2016). Effect of different seed priming on germination characteristics and some antioxidant enzymes activity of Echinacea purpurea. Iranian Journal of Seed Science and Research, 3(3), 125-135.  (in Persian)
5. Bagheri, M., Yeganeh, H., Esfahan, E.Z., & Savadroodbari, M.B. (2011). Effects of water stress on seed germination of Thymus koteschanus and Hohen and Thymus daenensis Celak. Middle East Journal of Scientific Research, 8(4), 726-731.
6. Chauhan, B.S., Gill, G., & Preston, (2006). Factors affecting seed germination of annual sowthistle (Sonchus oleraceus) in Southern Australia. Weed Science, 54(5), 854-860.‏ https://doi.org/10.1614/WS-06-047R.1
7. Chauhan, B.S., & Johnson, D.E. (2008). Germination ecology of Chinese sprangletop (Leptochloa chinensis) in the Philippines. Weed Science, 56(6), 820-825. https://doi.org/10.1614/WS-08-070.1
8. Chauhan, B., & Johnson, D.E. (2008). Seed germination and seedling emergence of giant sensitiveplant (Mimosa invisa). Weed Science, 56(2), 244-248.‏ https://doi.org/10.1614/WS-07-120.1
9. Dehkordi, R.M., Yadegari, M., & Hamedi, B. (2015). Effect of temperature, drought and salinity stresses on germination of Portulaca oleracea, Trigonella foenum-graecium L., Borago officinalis L. and Hypericum perforatum L. Advances in Environmental Biology, 9, 148-152.
10. Duan, L., Sebastian, J., & Dinneny, J.R. (2015). Salt-stress regulation of root system growth and architecture in Arabidopsis Plant Cell Expansion: Methods and Protocols, vol. 1242, 105-122.‏
11. Elahifard, E., Mijani, S., Kheyrandish, S., Kazerooni, E., & Tokasi, S. (2013). Investigation of dormancy and the effect of some environmental factors on germination of Junglerice (Echinocloa colona) seeds. Journal of Plant Protection, 27(3), 342-350. (in Persian with English abstract). https://doi.org/10.22067/jpp.v27i3.26762
12. Forieri, I., Hildebrandt, U., & Rostas, M. (2016). Salinity stress effects on direct and indirect defense metabolites in maize. Environmental and Experimental Botany, 122, 68-77. https://doi.org/10.1016/j.envexpbot.2015.09.007
13. Fricke, W. (2004). Rapid and tissue -specific accumulation of solutes in the growth zone of barley leaves in response to salinity. Planta, 219, 515-525. https://doi.org/10.1007/s00425-004-1263-0
14. Ghaderi, S., Ghorbani, J., Gholami, P., Karimzadeh, A., & Salarian, F. (2011). The effect of drought and salinity stress on the germination indices of (Vicia villosa). Journal of Agricultural Ecology, 3(1),121-130. (In Persian). https://doi.org/10.22067/jag.v3i1.9977
15. Ghahraman, A. (2004). Plant Systematic: Coromophytes of Iran. 1. Research Institute of Forests and Rangelands, Tehran, Iran. 520 p. (In Persian).
16. Hadi, H., Seyed Sharifi, R., & Namour, A. (2016). Phytoprotectants and Abiotic Stresses. Urmia University Publication, Iran. 341 p. (In Persian)
17. Halima, N.B., Ben Saad, R., Ben Slima, A., Khemakhem, B., Fendri, I., & Abdelkafi, S. (2014). Effect of salt stress on stress-associated genes and growth of Avena sativa L. Isesco Journal of Science and Technology, 10, 73-80.
18. Izadi, E., Zarghani, H, Mohammadian, M., & Yanegh, A. (2014). Evaluation of germination and seedling growth characteristics of three sesame (Sesamum indicum) cultivars in salt and drought stress condition. Agricultural Applied Research, 27(102), 92-100.‏ (In Persian with English abstract). https://doi.org/10.22092/aj.2014.100934
19. Jamali, F., & Etemad, V. (2022). Investigating different levels of salinity and drought stress on the germination and growth of Pennisetum divisum. Iranian Journal of Seed Science and Technology, 11(3), 89-102.‏ (In Persian with English abstract)  https://doi.org/10.22092/ijsst.2022.359459.1443
20. Kaya, M.D., Okcu, G., Atak, M., Cikili, Y., & Kolsarici, O. (2006). Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annus ). European Journal of Agronomy, 24(4), 291-295.‏ https://doi.org/10.1016/j.eja.2005.08.001
21. Koyro, H.W., & Eisa, S.S. (2008). Effect of salinity on composition, viability and germination of seeds of Chenopodium quinoa Journal of Plant and Soil, 302, 79-90. https://doi.org/10.1007/s11104-007-9457-4
22. Lotfifar, O., Allahdadi, I., Zand, E., Akbari, G.A., & Mottaghi, S. (2015). Effects of salinity and drought stresses due to Nacl and poly ethylene glycol on germination characteristics and seedling growth of wild mustard (Sinapis arvensis). Iranian Journal of Seed Science and Technology, 4(1), 97-108. (In Persian with English abstract)
23. Lynch, J., & Lauchli, A. (1988). Salinity affects intracellular calcium in corn root protoplasts. Plant Physiology, 87(2), 351-356. https://doi.org/10.1104/pp.87.2.351
24. Mazhari, M. (2013). Study and failure of weed seeds dormancy in Shahrekord region. M.Sc. Thesis, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran. 120 p. (In Persian).
25. McNeil, S.D., Nuccio, M.L., & Hanson, A.D. (1999). Betaines and related osmoprotectants. Targets for metabolic engineering of stress resistance. Plant Physiology, 120(4), 945-949. https://doi.org/10.1104/pp.120.4.945
26. Michel, B.E., & Kaufmann, M.R. (1973). The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51(5), 914-916. https://doi.org/10.1104/pp.51.5.914
27. Michel, B.E. (1983). Evaluation of the water potentials of solutions of polyethylene glycol 8000 both in the absence and presence of other solutes. Plant Physiology, 72(1), 66-70.‏ https://doi.org/10.1104/pp.72.1.66
28. Min Bashi Moini, M.,Esfandiari, H.,Pourazer, R.,Baghestani, M.A.,Zand, A.,Veisey, M., Sabeti, P.,Jamali, M.R.,Hatami, S.,Haghighi, A.,McNally, A.,Mousavi, K.,Nazerkakhki, H.,Narimani, V.,Noor Alizadeh, M., Valiallh-Pour, R.,& Norouzadeh, R. (2015). Evaluation of some problems of weed management in wheat fields in different regions of the country.Journal of Weed Knowledge, 11, 13-26.
29. Mojab, M., Zamani, G.H., Eslami, V., Hossieni, M., & Naseri, A. (2010). Effects of salinity and drought caused by different concentrations of sodium chloride and PEG 6000 on seed germination and seedling growth of this barnyardgrass (Echinocloa crus-galli Var: oryzicola). Journal of Plant Protection, 24(1), 108-114. (In Persian with English abstract)
30. Mozaffarian, V. (1994). Plant classification. Tehran: Contemporary Culture Publication, Iran.     960 p. (In Persian).
31. Munns, R., & Gilliham, M. (2015). Salinity tolerance of crops–what is the cost?. New Phytologist, 208(3), 668-673. https://doi.org/10.1111/nph.13519
32. Murshed, R., Lopez-Lauri, F., & Sallanon, H. (2014). Effect of salt stress on tomato fruit antioxidant systems depends on fruit development stage. Physiology and Molecular Biology of Plants, 20, 15-29. https://doi.org/10.1007/s12298-013-0209-z
33. Niazi, M. (2003). Study of some methods of breaking dormancy and germination in the seeds of some weeds. M.Sc. Thesis, Faculty of Agriculture, Shiraz University, Shiraz, Iran.
34. Okcu, G., Kaya, M.D., & , M. (2005). Effect of salt and drought stress on germination and seedling growth of pea (Pisum sativum L.). Turkish Journal of Agriculture and Forestry, 29(4), 237-242.‏
35. Parsa, A. (1980). Flora of Iran. Tehran: Tehran University Press, Iran. 1488 p. (In Persian).
36. Pirasteh-Anosheh, H. (2020). Breaking seed dormancy of camelthorn (Alhagi maurorum) using different treatments and salinity tolerance threshold level evaluation at germination stage. Iranian Journal of Seed Research, 7(1), 181-192.‏ (In Persian with English abstract). http://doi.org/10.29252/yujs.7.1.181
37. Pourhasan, F., Rashed Mohassel, M.H., & Izadi Darbandi, E. (2019). Effects of salinity and drought stresses due to Nacl and PEG 6000 on germination characteristics and seedling growth of rye (Secale cereale). In the First National Conference of Agricultural and Environmental Sciences of Iran. (In Persian)
38. Rahnama, A., James, R.A., Poustini, K., & Munns, R. (2010). Stomatal conductance as a screen for osmotic stress tolerance in durum wheat growing in saline soil. Functional Plant Biology, 37, 255-263. https://doi.org/10.1071/FP09148
39. Rashed Mohassel, M.H., Najafi H., & Akbarzadeh M.D. (2001). Weed Biology and Control. Publications of Ferdowsi University of Mashhad, Mashhad, Iran. 404 pages.
40. Sohrabi, S., Ghanbari, A., Mohassel, M.H.R., Mahalati, M.N., & Gherekhloo, J. (2013). Effect of desiccation and salinity stress on seed germination and initial plant growth of Cucumis melo. Planta Daninha, 31(4), 833-841. https://doi.org/10.1590/S0100-83582013000400009
41. Springer, T.L. (2005). Germination and early seedling growth of chaffy-seeded grasses at negative water Crop Science, 45(5), 2075-2080.‏ https://doi.org/10.2135/cropsci2005.0061
42. Taghvaei, M., & Ghaedi, M. (2014). The effect of osmotic conditions on germination of (Haloxylon aphyllum ) seeds and recovery under water stress. Agricultural Journal, 9(3), 167-172.
43. Tavakkol-Afshari, R., & Majnoun-Hossini, N. (2002). Responses of wheat and canola cultivars simulated drought conditions. In International Conference on Environmentally Sustainable Agriculture for Dry Areas for (Vol. 3).‏
44. Tester, M., & Davenport, R. (2003). Na⁺ tolerance and Na⁺ transport in higher plants. Annals of Botany, 91(5), 503-527.‏ https://doi.org/10.1093/aob/mcg058
45. Yadegari, M., & Shakerian, A. (2014). Irrigation Periods and Fe, Zn foliar application on agronomic characters of Borago officinalis, Calendula Officinalis, Thymus vulgaris and Alyssum desertorum. Advances in Environmental Biology, 8(4), 1054-1063.
46. Yadegari, M. (2014). Study of ratio and speed germination of twelve medicinal plants under several treatments of salinity. Advances in Environmental Biology, 8(4), 425-431.‏
47. Zadeh, H.M., & Naeini, M.B. (2007). Effects of salinity stress on the morphology and yield of two cultivars of canola (Brassica napus). Journal of Agronomy, 6, 409-414.
48. Zalnezhad, F. (2016). Investigation of ecophysiology of seed germination of (Gypsophila pilosa) Hudson. M.Sc. Thesis, Faculty of Agricultural Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
49. Zhang, Q., Rue, K., & Wang, S. (2012). Salinity effect on seed germination and growth of two warm-season native grass species. HortScience, 47(4), 527-530.‏ https://doi.org/10.21273/HORTSCI.47.4.527