Effect of Pretreatment of Sunflower (Helianthus annuus L.) Seed with Cold Plasma on its Yield and Yield Components in Competition with Weeds

Document Type : Research Article

Authors

1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran

2 Faculty of Physics, Shahrood University of Technology, Shahrood, Iran

3 Department of Biosystem Engineering, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran

Abstract

Introduction: Sunflower (Helianthus annuus L.) is a major worldwide oilseed crop that develops slowly during the initial weeks after planting. Weed competition with sunflower during the first four weeks after crop emergence can reduce sunflower yield significantly. Promoting seed germination is the most direct way to improve sunflower growth and competition with weeds. Per-treatment of seed by cold plasma through accelerating seed germination can increase competitive ability of plant with weeds. Cold plasma treatment significantly increased wheat growth and yields. Thus, increasing plant growth can improve competitive ability of it with weeds. The aim of this study was to investigate the effect of cold plasma treatment on growth and yield of sunflower in competition with weeds.
Materials and Methods: This experiment was carried out as factorial based on randomized complete block design with three replications at Shahrood University of Technology (latitude of 36° 25 'N and longitude of 54° 57' E with an elevation of 1345 m) in 2016. Experimental factors were including cold plasma at six levels: control, hydro-priming of seeds for 10 hours, pretreatment of seeds with cold plasma radiation for 15 and 30 seconds, hydro-priming of seeds for 10 hours + cold plasma radiation for 15 and 30 seconds and weed control at three levels: control (no weeding), weeding all season and application of trifluralin (1200 g. a.i. ha-1). Dielectric barrier discharge plasma jet was operated in ambient air under sterile conditions in the laboratory of Faculty of Physics, Shahrood University of Technology. After seed priming in distilled water for 10 hours, sunflower seeds were taken in petri plates and treated with the plasma for determined times. Seeds were planted in the field immediately after cold plasma treatment. Seed number per head, 100 seeds weight, harvest index, biological, grain and oil yield of sunflower and weed dry weight were recorded. Statistical analyses of data were performed with statistical software MSTATC. Significant differences between means refer to the probability level of 0.05 by LSD test.
Results and Discussion: The results showed that seed number per head, biological and grain yield, harvest index, oil yield and weed dry weight significantly affected by interaction effects of treatments. Results showed that biological, oil and seed yield of sunflower decreased by 62.76, 56.11 and 55.86% in weed-infest than weed-free treatments respectively. Seed number per head and biological yield of sunflower significantly increased by using different pretreatments methods than control in the weed-infest conditions. Also, pretreatment of seeds with cold plasma for 30 seconds increased oil and seed yield of sunflower by 68.8 and 58.5 percent respectively in the weed infest treatment than control. The plasma treatment of soybean seeds up to 2 min showed positive effects on their germination rate and seedling growth. Cold plasma has essential roles in a broad spectrum of developmental and physiological processes in plants, including reducing the bacterial bearing rate of seeds, changing seed coat structures, increasing the permeability of seed coats, and stimulating seed germination and seedling growth. In addition, plasma treatment also could improve the physiological metabolism of the plant, such as de-hydrogenase activity, superoxide dismutase and peroxidase activities, photosynthetic pigments, photosynthetic efficiency and nitrate reductase activity. It seems that hydro-priming technique and cold plasma irradiation with improving seed germination and seedling early growth can led to more and faster germination and better competition of sunflower plants with weeds. 
Conclusion: Based on our results, pre-treatment of seeds by cold plasma and hydro-priming in addition to reducing weed biomass, could significantly improve the growth characteristics and yield of sunflower through increasing crop competitive ability with weeds.

Keywords


  1. Abbasdokht H., Afshari H., Owji E., and Taheri S. 2016. The effect of seed priming and different levels of nitrogen application on quantitative and qualitative yield of sunflower progress cultivar. Crop Physiology Journal 8(29): 105-120. (In Persian with English abstract)
  2. Abbasdokht H., Makarian H., Ahmadi Sharaf H., Gholami A., and Rahimi M. 2012. The study of integrated weed management (IWM), emphasizing the effect of seed priming on yield and yield components of maize (Zea mayz L.). Weed Research Journal 2(4): 63-76. (In Persian with English abstract)
  3. Afzal I., Ahmad N., Basra S.M.A., Ahmad R., and Iqbal A. 2002. Effect of different seed vigor enhancement techniques on hybrid maize (Zea mays L.). Pakistan Journal of Agriculture Science 39: 109-112.
  4. Asghari J., Vahedi A., and KhoshghaulH.R. 2011. Critical period of weed control in sunflower (Helianthus annus L.) in west of Guilan province. Journal of Plant Protection 25(2): 116-126. (In Persian with English abstract)
  5. Basra S.M.A., Ashraf M., Iqbal N., Khaliq A., and Ahmad R. 2004. Physiological and biochemical aspects of pre-sowing heat stress on cottonseed. Seed Science and Technology 32: 765-774.
  6. Bayat S., and Sepehry A. 2012. Paclobutrazol and salicylic acid application ameliorates the negative effect of water stress on growth and yield of maize plants. Journal of Research in Agricultural Science 8(2): 127-139. (In Persian with English abstract)
  7. Caamal-Maldonado J.A., Jimenez-Osornio J.J., Torres-Barragan., and Anaya A.L. 2001. The use of allelopathic legume cover and mulch species for weed control in cropping systems. Journal of Agronomy 93: 27-36.
  8. Duma I .2006. Effect of seed priming with PEG and K3PO4 on germination and seedling growth in Lettuce. Pakistan Journal of Biological Science 9(5): 923-928.
  9. Deh Ahmadi A., Parsa S.R., Nezami M., and Ganjeali A. 2011. The effects of drought stress at different phenological stages on growth indices of chickpea (Cicer arietinum L.) in greenhouse conditions. Iranian Journal of Pulses Research 1(2): 69-84. (In Persian with English abstract)
  10. Ebrahimi F., Majnoun Hossieni N., and Hossirni M B. 2012. The effect of extracts of medicinal plants weed control (Amaranthus retroflexus L.) and (Chenopodium album L.) in beans. Iranian Journal of Field Crop Science 4: 757-765. (In Persian with English abstract)
  11. Elezovica I., Dattab A., Vrbnicaninc S., Glamoclijac S., Simicd M., Malidzae G., and Knezevic. S. Z. 2012. Yield and yield components of imidazolinone-resistant sunflower (Helianthus annuus L.) are influenced by pre-emergence herbicide and time of post-emergence weed removal. Field Crops Research 128: 137-146.
  12. Hiltbrunner J., Jeanneret P., Liedgens M., Stamp P., and Streit B. 2007. Response of weed communities to legume living mulches in winter wheat. Journal of Agronomy and Crop Science 193: 93-102.
  13. Hussain F., Iqbal M., Shah S.Z., Qamar M.A., Bokhari T.H., Abbas M., and Younus M. 2017. Sunflower germination and growth behavior under various gamma radiation absorbed doses. Acta Ecologica Sinica 37: 48–52.
  14. Jayasena D.D., Kim H.J., Yong H.I., Park S., Kim K., Choe W., and Jo C. 2015. Flexible thin-layer dielectric barrier discharge plasma treatment of pork butt and beef loin: Effects on pathogen inactivation and meat-quality attributes. Food Microbiology 46: 51-57.
  15. Kafi M. 2013. Biochemical response of two wheat cultivars (Triticum aestivum L.) to gamma radiation. Pakistan Journal of Botany 45: 473-477.
  16. Koga K., Thapanut S., Amano T., Seo H., Itagaki N., Hayashi N., and Shiratani, M. 2016. Simple method of improving harvest by nonthermal air plasma irradiation of seeds of Arabidopsis thaliana (L.). Applied Physics Express 9: 016201.
  17. Latify S., Yousefi A., and Jamshidi K. 2015. Effect of living mulch application on yield and yield components of Sunflower (Helianthus annuus L.) cultivars and weed control. Agricultural Science and Sustainable Production 25(2): 33-45. (In Persian with English abstract)
  18. Ling L., Jiafeng J., Jiangang L., Minchong S., Xin H., Hanliang S., and Yuanhua, D. 2014. Effects of cold plasma treatment on seed germination and seedling growth of soybean. Scientific Reports 4: 5859.
  19. Makarian H. 2002. Planting date and population density influence on competitiveness of corn (Zea mayz L.) with redroot pigweed (Amaranthus retroflexus L.). MSc Thesis, Ferdowsi University of Mashhad, Iran.
  20. Manarifard M., and Sepehri A. 2015. Effect of seed priming and foliar application of zinc on yield and yield components of two wheat cultivars. Journal of Agricultural Science and Sustainable Production 22(4.1): 151-165. (In Persian with English abstract)
  21. Mildažienė V., Aleknavičiūtė1 V., Žūkienė1 R., Paužaitė1 G., Naučienė1 Z., Filatova I., Lyushkevich V., Haimi P., Tamošiūnė I., and Baniulis D. 2019. Treatment of common sunflower (Helianthus annus L.) seeds with radio-frequency electromagnetic field and cold plasma induces changes in seed phytohormone balance, seedling development and leaf protein expression. Scientific Reports 9: 6437.
  22. Mohammadi G., Koohi Mohammad Abadi Y., Ghobadi M., and Najaphy A. 2015. Grain yield and weed control in corn influenced by seed priming and plant density. Journal of Agricultural Science and Sustainable Production 25(1): 13-28. (In Persian with English abstract)
  23. Moschner C.R., and Biskupek-Korell B. 2006.  Estimating the content of free fatty acids in high-oleic sunflower seeds by near- infrared spectroscopy. European Journal Lipid Science and Technology 108: 606-613.
  24. Nasiri Dehsorkhi A., Makarian H., Gholipoor M., and Abbasdokht H. 2017. Investigate effect of ultrasonic waves and seed priming on emergence and growth of cowpea (Vigna sinensis) under soil application of trifluralin. Journal of Plant Protection 31(1): 40-51. (In Persian with English abstract)
  25. Olatunde O.O., Benjakul S., and Vongkamjan K. 2019. Dielectric barrier discharge cold atmospheric plasma: Bacterial inactivation mechanism. Journal of Food Safety e12705.
  26. Pak Mehr A., Rastgoo M., Shekari F., Saba J., Vazayefi M., and Zangani E. 2011. Effect of Salicylic acid priming on yield and yield components of cowpea (Vigna unguiculata L.) under water deficit at reproductive stage.  Iranian Journal of Pulses Research 2(1): 53-64. (In Persian with English abstract)
  27. Santini B.A., Rojas-Aréchiga M., and García Morales E. 2017. Priming effect on seed germination: Is it always positive for cacti species? Journal of Arid Environments 147: 155-158.
  28. Sera B., Spatenka P., Sery M., Vrchotova N., and Hruskova I. 2010. Influence of plasma treatment on wheat and oat germination and early growth. IEEE Transactions on Plasma Science 38: 2963–2968.
  29. Sera B., Stranak V., Sery M., Tichy M., and Spatenka P. 2008. Germination of Chenopodium album in response to microwave plasma treatment, Plasma Science Technolory 4: 506-511.
  30. Stoffels E., Sakiyama Y., and Graves D.B. 2008. Cold atmospheric plasma: charged species and their interactions with cells and tissues. IEEE Transactions on Plasma Science 36: 1441–1451.
  31. Thirumdas R. 2018. Exploitation of cold plasma technology for enhancement of seed germination. Journal of Agricultural Research and Technology 13(2): 1-5.
  32. Zand E., Bena Kashani F., Baghestani M.A., Maknali A., Minbashi M., and Soufizadeh S. 2007. Investigating the distribution of resistant wild oat (Avena ludoviciana) populations to clodinafop-propargil herbicide in south western Iran. Environmental Science 3: 85-92. (In Persian with English abstract)
  33. Yousefi A., and Anjil Eli M. 2013. Weed management in sunflower using cover crops. Agricultural Science and Sustainable Production 9: 69-77. (In Persian with English abstract)
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