Investigating the Resistance of Winter Wild Oat Biotypes to ACCase Inhibitor Herbicide in Wheat Fields of Ramian County

Document Type : Research Article

Authors

• M. Heravi
• J. Gherekhloo
• A. Siahmarguee
• H. Kazemi
• S. Hassanpour Bourkheili

Gorgan University of Agricultural Sciences and Natural Resources

10.22067/jpp.v34i3.86187

Abstract

Introduction: Weeds are wild plant species which grow in agricultural ecosystems and compete with crops for resources such as water, nutrients, light, and space. Wild oat is reported as one of the most troublesome autumn weeds in croplands and its dominant species in Iran is winter wild oat (Avena ludoviciana Dur.). Herbicide resistance is the inherent ability of a weed biotype in survival after being exposed to a rate of herbicide which would be lethal to the wild type species. In the recent years, there have been several reports on the occurrence of herbicide resistance in Golestan province. The following research was conducted to identify the ACCase- resistant winter wild oat biotypes and generation of distribution map for the resistant biotypes in wheat fields of Ramian Township.
Materials and Methods: The present study was conducted in 2017-2018 at the greenhouse and Weed Science laboratory of Gorgan University of Agricultural Sciences and Natural Resources. Plant material included 80 putatively resistant winter wild oat biotypes which were collected from 100 wheat fields of Ramian Township. The susceptible biotype was also collected from the regions with no history of herbicide spray. Three Acetyl coenzyme A carboxylase (ACCase) inhibiting herbicides including clodinafop propargyl, fenoxaprop-P ethyl, and diclofop methyl which are common graminicides applied in wheat fields of the country were used to confirm the occurrence of resistance in winter wild oat biotypes. Three herbicides including pinoxaden, clethodim, and haloxyfop-R methyl ester were also applied on the biotypes as the possible alternative herbicides. First the winter wild oat seeds were hulled by hand. To obtain a more uniform germination, the seeds were placed in 9 cm Petri dishes topped with a filter paper and 2.5 mL of distilled water was added to the Petri dishes. Then, the Petri dishes were transferred to a refrigerator with a temperature of 4-5°C under darkness conditions. After pre-chilling, the petri dishes were incubated at the room temperature to germinate. To perform the discriminating concentration, pre- screening and concentration- response assays, 10 pre- germinated seeds from each biotype were placed on the Petri dishes as described above, then were treated with various concentrations of the mentioned herbicides. Three Petri dishes were used for each concentration, with each Petri dish serving as a replicate. Also, the Petri dishes treated with distilled water were regarded as control. The Petri dishes were kept at the room temperature for seven days and then the length of their coleoptile was measured. Four parametered log-logistic function was fitted to the data using R software (drc package). ArcGis V.10.3 was used to generate the distribution map of the herbicide- resistant biotypes.
Results and Discussion: Results of the present study indicate the confirmation of resistance to clodinafop propargyl, fenoxaprop-P ethyl, and diclofop methyl herbicides in 58 out of 80 winter wild oat biotypes gathered from wheat fields of Ramian Township. Resistance factor to clodinafop propargyl, fenoxaprop-P ethyl, and diclofop methyl herbicides were 48.69 to >851.33, 52.57 to >752.57, and 310.27 to >1443.90, respectively. For further investigation on the response of these resistant biotypes, three herbicides including pinoxaden, clethodim, and haloxyfop-R methyl ester were applied on the biotypes as a Petri dish assay. Two biotypes with the highest resistance factors (ram1, ram18) and two with the lowest resistance factors (ram15, ram4) were selected for this assay. In the haloxyfop-R methyl ester treatment, the highest and lowest resistance factors were observed in ram1 with 423.35 and ram15 with 284.50, respectively. In the other two herbicide however, resistance factor had no significant difference with the value 1.  Application of herbicides possessing different modes of action may lead to elimination of both susceptible and resistant biotypes. However, this will serve as a new selective pressure which will eventually result in intensification of resistance and furthermore, evolution of cross and multiple- resistant species. Thus, meticulous application of integrated weed management methods are of great importance to prevent or delay the evolution of resistance to herbicides.
Conclusion: Implementation of cultural methods for weed management and preventing the distribution of herbicides to which the weeds have readily developed resistance will lead to reduction of selective pressure. Adoption of crop and herbicide rotation principles will serve as a tool to debilitate the weed in competition with the crop, which in long term may contribute to reduced frequency of resistant alleles.

Keywords

• Concentration-response assay
• Distribution map
• Herbicide resistance
• Resistance factor

20.1001.1.20084749.1399.34.3.10.2

Main Subjects

• Weed

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References

1- Banakashani F., Zand E., and Alizadeh H.M., 2007. Resistance of wild oat (Avena ludoviciana) biotypes to clodinafop-propargil herbicide. Applied Entomology and Phytopathology 74: 127-149. (In Persian with English abstract)

2- Bourgeois L., and Morrison I.N. 1997. Mapping risk areas for resistance to ACCase inhibitor herbicides in Manitoba. Canadian Journal of Plant Science 77: 173-179.

3- Burke I.C., Thomas W.E., Burton J.D., Spears J.F., and Wilcut J.W. 2006. A seedling assay to screen aryloxyphenoxypropionic acid and cyclohexanedione resistance in johnsongrass (Sorghum halepense). Weed Technology 20(4): 950-955.

4- Gherekhloo J., Osuna M.D., and De Prado R. 2012. Biochemical and molecular basis of resistance to ACCase‐inhibiting herbicides in Iranian Phalaris minor populations. Weed Research 52: 367-372.

5- Gherekhloo J., Oveisi M., Zand E., and De Prado R. 2016. A review of herbicide resistance in Iran. Weed Science 64:551-561.

6- Heap I. 2020. International survey of herbicide resistance weeds. www.weedscience.com. Accessed: 10 Jan 2019.

7- Kalami R. 2014. Identification of resistant weeds to ACCase and ALS inhibitors in wheat fields of Kordkuy. MSc Thesis, Gorgan University of Agricultural Sciences and Natural Resources. (In Persian)

8- Koller M., and Lanini W.T. 2005, Site-specific herbicide applications based on weed maps provide effective control. California Agriculture 59: 182-187.

9- Maneechote C., Preston C., and Powles S.B. 1997. A diclofop‐methyl‐resistant Avena sterilis biotype with a herbicide‐resistant acetyl‐coenzyme a carboxylase and enhanced metabolism of diclofop‐methyl. Pesticide Science 49: 105-114.

10- Maxwell B.D., Roush M.L., and Radosevich S.R. 1990. Predicting the evolution and dynamics of herbicide resistance in weed populations. Weed Technology 4: 2-13.

11- Montazeri M., Zand E., and Baghestani M.A. 2005. Weeds and their control in wheat fields of Iran. Advances in Agronomy 58: 57-93.

12- Mueller T.C., Mitchell P.D., Young B.G., and Culpepper A.S. 2005. Proactive versus reactive management of glyphosate-resistant or -tolerant weeds. Weed Technology 19(4): 924-933.

13- Najjari Kalantari. 2013. Identification of resistant weeds to ACCase and ALS inhibitors in wheat fields of Aq Qala and preparing their distribution map. MSc Thesis, Gorgan University of Agricultural Sciences and Natural Resources. (In Persian)

14- Prather T.S., Ditomaso J.M., and Holt J.S. 2000. Herbicide resistance, definition and management strategies. Publication of Division of Agriculture and Natural Resources. UC Davis USA. Publication 8012.

15- Rastgoo M. 2007. Detecting of Avena ludoviciana resistant to aryloxyphenoxypropionate herbicides in wheat fields of Khuzestan province. PhD Thesis, Ferdowsi University of Mashhad. (In Persian)

16- Razghandi A. 2016. Identification of resistant biotypes to aryloxy phenoxy propionate and asetolactate synthase inhibitors in wheat fields of Aliabad-e katool and preparing their distribution map. MSc Thesis, Gorgan University of Agricultural Sciences and Natural Resources. (In Persian)

17- Retrum J., and Forcella F. 2002. Giant foxtail (Seteria faberi) seedling assay for resistance to sethoxydim. Weed Technology 16(2): 464-466.

18- Ritz C., and Streibig J.C. 2005. Bioassay analysis using R. Journal of Statistical Software 12(5): 1-22.

19- Soofizadeh T. 2015. Identification of resistant biotypes to aryloxy phenoxy propionate and asetolactate synthase inhibitors in wheat fields of Kalaleh and preparing their distribution map. MSc Thesis, Gorgan University of Agricultural Sciences and Natural Resources. (In Persian)

20- Tatari S., Gherekhloo J., Siahmarguee A., and Kazemi H. 2018. Identification of resistant Avena ludoviciana Dur accessions to ACCase inhibitor herbicides in Gonbad-E Kavus wheat fields and mapping their distribution. Plant Productions 41(2): 103-116.

21- Zand E., and Baghestani M.A. 2002. Weed resistance to herbicides. Jihad-e-Daneshgahi Press, 176pp.

22- Zand E., Baghestani M., Nezamabadi N., and Shimi P. 2017. Application guide of registered herbicides in Iran. Jihade-e-Daneshgahi Press, Mashhad, 223 pp.

23- Zhang Y., Li W., Zhou W., Jia H., Liu L., Li B., Han Y., and Qi S. 2020. Dissipation dynamics and dietary risk assessment of pyraclonil residues in rice (Oryza sativa L.). Microchemical Journal 152: 104440.