(Brief report) Insecticidal Efficacy of Different Formulations of Diatomaceous Earth on Tribolium castaneum (Herbst) (Col., Tenebrionidae) Adults in Lab Conditions

Document Type : Research Article


1 Islamic Azad University of Arak

2 Iranian Research Institute of Plant Protection


The insecticidal efficacy of diatomaceous earth, formulation Sayan® was evaluated against the red flour beetle adults, Tribolium castaneum (Herbst) (Col., Tenebrionidae), in comparison with 3 commercially DE formulations (PyriSec®, SilicoSec® and Insecto®) in laboratory conditions (25±1C°, 65±5% R.H. in dark). The effect of different formulation of DE with different doses (100, 250, 500, 1000 and 1500 ppm)were usedon 7days adults of T. castaneum at four replicates and the adult mortality was recorded after 1, 2, 3 and 7 days post-treatments. The results showed that there were significant differences among different formulation of DE and also different doses at any exposure intervals. The adult mortality increased with increasing doses and exposure times in all DEs. For Sayan®, highest and lowest mortality were 96 and 3.3% after 7 and 1days of exposure at 1500 and 100 ppm doses. There was significant difference among 4 DE formulations, so PyriSec® had highest and Sayan® had lowest effect on adults mortality: the highest to the lowest effectiveness was being: PyriSec® > SilicoSec® >Insecto® > Sayan®


Agrinova Company. 2012. Agrinova Biologische Präparate Produktions-und Vertriebs GmbH, Germany. Available on: http://www.agrinova.de/cms/produkte/schaedlings-und-liegenbekaempfung/
2- Arnaud, L., Tin Lan, H. T., Brostaux, Y. and Haubruge, E. 2005. Efficacy of diatomaceous earth formulations admixed with grain against populations of Tribolium castaneum. Journal of Stored Products Research, 41(2):121-130.
3- Arthur, F. H. 2000. Toxicity of diatomaceous earth to red flour beetles and confused flour beetles (Coleoptera: Tenebrionidae): effect of temperature and relative humidity. Journal of Economic Entomology, 93: 526-532.
4- Arthur, F. H. 2004. Evaluation of a new insecticide formulation (F2) as a protectant of stored wheat, maize and rice. Journal of Stored Products Research, 40, 317-330.
5- Athanassiou, C. G., Vayias, B. J., Dimizas, C. B., Kavallieratis, N. G., Papagregoriou, A. S. and Buchelos, C. T. 2005. Insecticidal efficacy of diatomaceous earth against Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Tribolium confusum du Val (Coleoptera: Tenebrionidae) on stored wheat: influence of dose rate, temperature and expoture interval. Journal of Stored Products Research, 41, 47-55.
6- Athanassiou, C. G., Arthur, F., Opit, G. P. and Throne, J. E. 2009. Insecticidal effect of diatomaceous earth against three species of stored-product psocids on maize, rice and wheat. Journal of Economic Entomology. 102(4): 1673-1680.
7- Bagheri-Zenouz, E. 1986. Les animaux nuisibles aux produits entreposès. Vol.1. Les Coleopteres depredateures de produits alimentaires et industriels. Sepehr Pub. Tehran. Iran. 309 p. [In Persian].
8- Casida, J. E. 1973. Pyrethrum, the natural insecticide. Academic Press. INC. 332p.
9- De Paula, M. C. Z., Flinn, P. W., Subramanyam, Bh. and Lazzari, M. N. 2002. Effects of age and sex on mortality of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) exposed to INSECTO®-treated wheat. Journal of the Kansas Entomological Society, 75(3): 158-162.
10- Dowdy, A. k. and Fields, P. G. 2002. Heat combined with diatomaceous earth to control the confused flour beetle (Coleoptera: Tenebrionidae) in flour mill. Journal of Stored Products Research. 38, 11-22.
11- Fields, P. and Korunic, Z., 2000. The effect of grain moisture content and temperature on the efficacy of diatomaceous earths from different geographical locations against stored-product beetles. Journal of Stored Products Research 36, 1–13.
12- Golestan-Hashemi, F., Farazmand, H., Karimzadeh, J. and Marouf, A. 2011. Effect of Iranian formulation of diatomaceous earth on confused flour beetle, Tribolium confusum Duval (Col.: Tenebrionidae), under laboratory conditions. Journal of Entomological Research. 2(4): 307-317. [In Persian with English abstract]
13- Golob, P. 1997. Current status and future perspectives for inert dust for control of stored products. Insect Journal of Stored Product Research, 33: 69-79.
14- Ghasemzadeh, M., Shakhsi Zare. F., Sabetghadam, A. and Baghaei. N. 2013. Integrated effects of diatomaceous earth and corn genotypes on mortality of red flour beetle Tribolium costaneum (Herbst) (Col., Tenebrionidae) in laboratory conditions. Journal of Entomological Research, 4: (1): 43-50. [In Persian with English summary].
15- Junior, A. L. M., Junior, M. M., Pereira, P. R. V. da S. & .de Paiva, W. R. S. C (2006). Effectivness of different dosages of diatomaceous earth to control Tribolium castaneum (Coleoptera: Tenebrionidae) in corn stored in the state of Roraima. 9th International Working Conference on store product protection. 1269- 1273.
16- Kabir, B. G. J., Lwan, M.and Gambo, F. M. 2011. Efficacy and persistence of raw diatomaceous earth against Tribolium castaneum (Col.: Tenebrionidae) on stored maize sorghum and wheat. Academic Journal of Entomology, 4(2): 51-58.
16- Kimiasabzavar Company. Iran. 2010. Available on: http://www.kimiasabzavar.com.
18- Korunic, Z. and Fields, P. G. 1995. Diatomaceous earth insecticidal composition. Canadian and U.S.A. Patents Pending.
19- Korunic, Z. and Rozman, V. 2010. A synergistic mixture of diatomaceous earth and deltamethrin to control stored grain insects. 10th International Working Conference on Stored Product Protection. July 2010, Portugal, 894-898.
20- Nabaei, N., Mehrvar, A., Saber, M. and Bagheri, M. 2012. Efficacy of entomopathogenic fungi in combination with diatomaceous earth against Callosobruchus maculates (Coleoptera: Bruchidae). Acta Entomologica Sinica. 55(11): 1282-1288.
21- Rezaei Torshizi, H. R., Farazmand, H., Goldasteh, S. & .Marouf, A. 2011. Effect of temperature on the toxicity of Iranian formulation of diatomaceous earth on bruchid beetle, Callosobruchus maculates F (Col., Bruchidae). Proceeding of the second Iranian pest management conferences. Shahid Bahonar Uni. Kerman, Iran, pp. 101
22- Rigaux. M., Haubruge. E. & .Fields. P. G (2000). Mechanisms for tolerance to diatomaceous earth between strain of Tribolium castaneum. Entomologia Experimentalis et Applicata, 101: 33-39
23- Shakhsi Zare, F., Ghasemzadeh, M., Baghaei N., Sabetghadam, A. and Nikpei, A. 2012. Mortality of red flour beetles, Tribolium castaneum (Herbst), feeding on three different hybrids of corn combined with diatomaceous earth (INSECTO®) in laboratory conditions. 20th Iranian Plant Protection Congress. 25-28 Aguest, 2012, Shiraz, Iran, p. 715
24- Shakhsi Zare, F., Ghasemzadeh, M., Sabetghadam, A. and Baghaei, N. 2012. Integrated effects of diatomaceous earth and Gamma ray on mortality of Red Flour beetle Tribolium castaneum (Col.: Tenebrionidae) in laboratory conditions. 20th Iranian Plant Protection Congress. Shiraz, Iran, pp 328.
25- Shakhsi Zare, F. 2013. Efficacy of Iranian formulation of diatomaceous earth, kaolin and two fungal isolates of Metarhizium anisopliae Sorokin in control of two stored product pests under laboratory conditions. Ph.D. dissertation, Islamic Azad University of Arak, 144 p.
26- Shayesteh. N. and Ziaee. M. 2007. Insecticidal efficacy of diatomaceous earth against Tribolium castaneum (Herbst) (Col: Tenebrionidae). Caspian Journal of Environmental Science, 5(2): 119-123.
27- Subramanyam, Bh. and Roesli, R. 2000. Inert dusts. In: Subramanyam, Bh., Hagstrum, D.W. (Eds.), Alternatives to Pesticides in Stored-Product IPM. Kluwer Academic Publishers, Dordreecht, pp. 321–380.
28- Subramanyam, B., Swanson, C.L., Madamanchi, N. and Norwood, S. 1994. Effectiveness of InsectoTM, a new diatomaceous earth formulation, in suppressing several stored-grain insect species. In: Highley, E., Wright, E.J., Banks, H.J., Champ, B.R. (Eds.), Proceedings of the Sixth International Working Conference on Stored-Product Protection, 17–23 April 1994, Canberra, Australia. CAB International, Oxford, UK, pp. 650–659.
29- Vayias, B. J. and Arthanassiou, C. G. 2004. Factors affecting efficacy of the diatomaceous earth formulation SilicoSec against adults and larvae of the confused beetle Tribolium confusum du Val (Coleoptera: Tenebrionidae). Journal of Crop Protection, 23: 565-573.
30- Ziaee, M. and Khashaveh. A. 2007. Effect of five diatomaceous earth formulations against Tribolium castaneum (Col:Tenebrionidae), Rhyzoptera dominica (Col:Bostrychidae) and Oryzaephilus surinamesis (Col:Silvanidae). Journal of Insect Sciences, 14: 359-365.