Evaluation of Tomato Cultivars Resistance to Root-knot Nematode (Meloidogyne javanica) in Greenhouse Conditions

Document Type : Research Article

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Abstract

Root-knot nematodes (Meloidogyne spp.) are one of the most important parasitic nematodes of tomato in the world and Iran as well. Meloidogyne javanica is the most widespread nematode in the tomato fields of Khorasan Razavi province. In order to evaluate the resistance, 12 tomato cultivars with two initial inoculation level of 5000 and 15000 eggs and larva of nematode in 1 kg soil have been used. The following parameters such as gall index, number of egg masses, final population, tomato root and stem weight were determined. In final rating of cultivars reaction, the two factor, gall index (GI) and reproduction factor (RF) were used. Result showed that Mobil cultivar was resistance in two levels of infection. Royal RS and King Rock cultivars showed hypersensitive reaction when infected by 5000 egg and larvae of nematode in the soil but they were susceptible while inoculation level increased to 15000 eggs and larva. The other cultivars were susceptible. In order to determine changes of total phenol level in tomato cultivars roots, 4 cultivars were selected. Sampling were daily measured by spectrophotometer within 12 days. Then total phenol calculated at microgram /gram root. Results indicated that total phenol of tomato cultivars and their blanks were significantly different (p≤0.05) with control. The total phenol noted in resistance varieties was more than susceptible varieties.

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