Cloning of luxAB Reporter Gene into Iranian Plant Pathogen Bacterial Isolates Pseudomonas syringae and Ralostonia solanacerum

Document Type : Research Article

Authors

S. Mostofi

M. Mashreghi

M. Bahreini

F. Oroojalian

P. Pordeli

10.22067/jpp.v25i4.11864

Abstract

Abstract
The ability to monitor particular microorganism in an environment is a difficult task. Many genes permit the differentiation of strains by conferring production of unique phenotypes such as bioluminescence [marine bacterial luciferase (luxAB)]. Therefore, lux-AB gene was cloned into two endemic plant pathogens Pseudomonas syringae and Ralostonia solanacearum by electrotransformation. lux-marking of above strains was carried out using miniTn-5 luxAB transposon. Purified strains were transformed with plasmid pUT containing luxAB gene by electroporator. Electroporation was performed in voltage 2/5 KV for 5 milisecend. All luxAB marked strains could grow on KB agar medium containing 12.5 µg/ml tetracycline and their luminescence intensity was measured by luminometer. lux-marked P. syringae and R. solanacearum were stable genetically engineered strains making them quite appropriate.

Keywords: Pseudomonas syringae, Ralostonia solanacerum, luxAB gene, strain monitoring

20.1001.1.20084749.1390.25.4.11.2

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