Investigation biological control two strains of Antifungal Actinomycetes against Phytophthora parasitica and P. citrophthora in vitro and in vivo conditions

Document Type : Research Article


Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol


this research was invistigated activitiy of antagonistic activity of 200 soil Actinomycete isolates assayed against Phytophthora parasitica and P. citrophthora in Kerman Provice. Among all Actinomycetes, strains of 19 and 29 showed high level of activity in Agar disk and Well-diffusion methods. In both isolates, the active substance was water and metanol soluble but insoluble in chloroform. The investigation Greenhouse showed effects of four treatments pathogen, pathogen by antagonist ,antagonist and control on high and dry wet root seedlings and length , wide leaves were significantly different at level 0/05% in completely randomized experimental design. Strin 19 on high and dry wet root seedlings and lenth leaves isolate and Strin 29 on wide leaves were most effective.From the results of our studies it is clear that usage of strain 19 and 29 as a biofungicidal natural product for application as an amendment in greenhouse soil mix inhibits or reduces the pathogen adverse effects. Antagonistic activity of two isolates in vivo confirm activity of biocontrol them in Greenhouse experiments.

Keywords: Citrus gumusis, Phytophthora parasitica, P. citrophthora Actinomycetes,Biological control