Molecular Analysis of Six Beet yellows virus (BYV) Isolates from West Azarbayjan, Kermanshah and Hamedan Provinces of Iran Based on ORF CP

Document Type : Research Article

Authors

• Mohadese Gerami nooghabi
• Mohsen Mehrvar ¹
• Atefe Hosseini ²

¹ Ferdowsi University of Mashhad

² University of Birjand

10.22067/jpp.v28i4.28061

Abstract

Beet yellows virus (BYV) belongs to the genus Closterovirus, Closteroviridae family, is one the most important viruses of sugar beet worldwide. In order to identify and characterize some molecular aspects of the BYV, a total of 75 symptomatic leaf samples of sugar beet were collected from sugar beet field of West Azarbayjan, Hamedan and Kermanshah provinces, Iran. Leaves showing general yellowing, vein clearing and thickness were collected. The primary detection of BYV performed by DAS-ELISA, using specific antibodies raised against coat protein of the virus, resulted in positive reaction of six out of 75 samples. The coat protein ORF of BYV isolates was amplified by RT-PCR, using BYV-F/BYV-R primer pair. Amplified fragments in expected size of 615 bp were directly sequenced. All sequence data were multiple aligned with other BYV isolates (from NCBI) using DNAMAN 7 software. An Ukrainian isolate (X73476) showed the highest nucleotide (99.19%) and amino acid (97.55%) identity with our isolates in this study. Phylogenetic analyses were conducted by MEGA 5.2. The results of phylogenetic analysis showed that BYV Iranian isolates and BYV Ukrainian isolate are clustered together.

Keywords

• Beet yellows virus
• Sugar beet
• western Azarbayejan
• Kermanshah and Hamadan and cp

20.1001.1.20084749.1393.28.4.18.0

---

References

1- آمارنامه کشاورزی، 1390-1389، دفتر آمار و فن آوری وزارت جهاد کشاورزی

2- ارجمند م.ن، 1377، بیماری‌های چغندر قند(442-357)، در"چغندر از علم تا عمل" تالیف دی.ا.کوک و اسکات، چاپ اول، نشر علوم کشاورزی، تهران.

3- اسکندری ف.، آل آقا ن.، حجارود ق. و همتی ک. 1348، بیماری‌های چغندرقند در ایران. نشریه شماره 106 گیاهپزشکی، دانشکده کشاورزی، دانشگاه تهران.

4- ذبیح نیا مقدم ع.، جعفرپور ب.، فلاحتی رستگار م. و گرایلی ن. 1389، بررسی آلودگی و تعیین پراکنش ویروس زردی چغندرقند (BYV) در مزارع خراسان رضوی، حفاظت گیاهان جلد 24، شماره 3، 257-252.

5- ایزدپناه ک. 1361. لیست مشروح بیماری های ویروسی و شبه ویروسی در فارس، جهاد دانشگاهی شیراز، شیراز.

6- Adams M.J., Antoniw J.F., and Fauquet C.M. 2004. Molecular criteria for genus and species discrimination within the family Potyviridae. Archive of Virology, 150:459–479.

7- Agranovsky A.A., and Lesemann D.E. 2000. Beet yellows virus. CMI/AAB Descriptions of Plant viruses: No. 377.

8- Clark M.F., and Adams A.N. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Genetic virology, 34: 475-483.

9- Dolja V.V., Boyko V.P., Agranovsky A.A., and Koonin E.V. 1991. Phylogeny of capsid proteins of rod-shaped and lamentous RNA plant viruses two families with distinct patterns of sequence and probably structure conservation. Virology 184, 79±86.

10- Dolja V.V., Kreuze J.F., and Valkonen J.P.T. 2006. Comparative and functional genomics of closteroviruses. Virus Research, 117: 38–51

11- Dolja V.V. 2003. Beet yellows virus: the importance of being different. Molecular Plant Pathology 4: 91-98.

12- Duffus J.E. 1973. The yellowing virus diseases of Beet. Virus Research 8: 347-386

13- Erokhina T.N., Zinovkin R.A., Vitushkina M.V., Jelkmann W., and Agranovsky A.A. 2000. Detection of Beet yellows closterovirus methyltransferase-like and helicase-like proteins invivo using monoclonal antibodies. Genetic Virology, 81, 597–603.

14- Esau K. 1960. The development of inclusions in sugar beets infected with the beet yellows virus. Virology. 11: 317.

15- Esau K. Cronshaw J., and Hoefert L. 1967. Relation of Beet yellows virus to the phloem and to movement in the sieve tube. Cell Biology, 32: 71-87.

16- Gushchin V.A., Solovyev A.G., Erokhina T.N., Morozov S.Y., and Agranovsky A. 2013. Beet yellows virus replicase and replicative compartments: parallels with other RNA viruses. Microbiology, 4:1-6.

17- Koeijer K.J., and Werf V.W. 1999. Effects of Beet yellows virus and Beet mild yellowing virus on leaf area dynamics of sugar beet (Beta vulgaris L.). Field Crops Research, 61: 163-177.

18- Rubio L., Guerri J., and Moreno P. 2013. Genetic variability and evolutionary dynamics of viruses of the family Closteroviridae. Microbiology, 4.

19- Russell G.E., Bell J. 1963. The structure of Beet yellows virus filaments. Virology, 21: 283-284.

20- Smith H.G., and Karasev A., 1991. Homepage of Plant Viruses Online, Descriptions and Lists from the VIDE Database. Beet yellowsclosterovirus Available online at http://image.fs.uidaho.edu/vide/descr093.htm.

21- Steven S.M., Hull R., Smith HO. 1997. Comparison of ELISA and RT-PCR for the detection of Beet yellows closterovirusin in plant and aphids. Virology Methods, 68:9–16.

22- Sylvester E.S. 1956. Beet yellows virus transmission by the green peach aphid. Economic Entomology 49, 789-800.

23- Tabejamaat Z., Massah A., and Ahoonmanesh A. 2013, Molecular Analysis of ORF6, ORF7 and ORF8 of Beet yellows virus Iranian Isolate. Phytopathology, 161: 574–577.

24- Watson M.A. 1940. Studies on the transmission of sugar Beet yellows virus by the aphid, Myzuspersicae. Proceeding of the Royal Society 128:535-52.

25- Zhu H.Y., Ling K.S., Goszczynski D.E., McFerson J.R., and Gonsalves D. 1998. Nucleotide sequence and genome organization of grapevine leafroll-associated virus-2 are similar to Beet yellows virus, the closterovirus type member. Genetic Virology, 79, 1289±1298.