Biological Effects of Trichoderma spp. Isolated from Tomato Rhizosphere and Trichomax-HV Biofungicide against Tomato Fusarium Wilt in Vitro

Document Type : Research Article

Authors

• S. Jalali ¹
• M. Darvishnia ²
• N. Panjekeh ¹
• S. Pakbaz ²

¹ Department of Plant Protection, Faculty of Agriculture, Zabol University, Sistan & Balouchestan, Iran

² Department of Plant Protection, Faculty of Agriculture, Lorestan University, Lorestan, Iran

10.22067/jpp.2021.32846.0

Abstract

Introduction: Fusarium wilt of tomato is one of the most important and common tomato diseases growing in the cultivated areas of this plant around the world. Biological control of plant diseases using non-pathogenic microorganisms has attracted the attention of many researchers. Trichoderma is one of the most successful and widely used microorganisms that prevent fungal damage in plants. Trichoderma spp. have different mechanisms to deal with plant pathogens, such as increasing plant resistance and activating defense reactions, direct confrontation with the pathogen through mycoparasitism, antibiosis, competition, plant growth stimulation, regulation and induction of plant growth factors including auxins, cytokinins and ethylene that stimulate growth Plants are involved. Totally, they destroyed biological reproductive organs, survival and reproduction, weakening and expelling pathogens from plant debris and preventing the formation of contaminants are the three basic methods by which biological control is achieved. Trichoderma is one of the living fungi that can perform the above three mechanisms simultaneously and as a result it is used as a biological fungus that increases plant growth and activity of beneficial microorganisms.
Material and Methods: In this study, the effects of two isolates; Trichoderma harzianum and Trichoderma virens, and biological toxin of Trichomax-HV against Fusarium oxysporum f. sp. lycopersici which is the agent of Fusarium wilt disease in tomato was evaluated in the laboratory and greenhouse. Trichomax-HV is a biofungicide that use as commercially. During summer and autumn, tomato plants with obvious signs of Fusarium wilt were collected from tomato fields in different parts of Lorestan province and transferred to the laboratory. Isolation of pathogenic fungi was performed according to Bani Hashemi and Dezeeuw methods using acidic PDA culture medium. Antagonistic activity of these isolates against this pathogen was studied by dual culture method in the laboratory. In order to investigate the effect of these antagonist isolates in the greenhouse, first the inoculum of pathogenic fungus and fungal antagonists were added to the pots as soon as the seedlings were transferred to the pots and then after 60-days, the interactions between the pathogen and antagonist was evaluated. This test was performed in a completely randomized design with 10 treatments and three replications. The data obtained from the experiment were statistically analyzed using SPSS 16 software and the average of the treatments were compared using Duncan's multiple range test at 5% probability level.
Results and Discussion: In this study, a total of three isolates of F. oxysporum f. sp. lycopersici were isolated from tomato plants infected with Fusarium wilt disease from Robat and Chegeni Dore tomato fields (Khorramabad city) and Cham fig fields (Poldokhtar city) that was assigned them codes FOM, FOK and FOS respectively. A total of 27 fungal isolates were isolated from soil samples of tomato rhizosphere collected from tomato fields of two cities in Lorestan province. By evaluating the inhibitory effect of these isolates on pathogenic growth, two isolates prevented the growth of pathogenic mycelium by creating an inhibitory aura. The results of laboratory studies showed that T. harzianum by 48.9 percent, Trichomax-HV by 45.6 percent and‏ T. virens to 22.36 percent prevented the growth of pathogen and colonized the pathogen’s colonies (in comparison with the control). Antagonist agents in greenhouse experiments increased stem height, wet and dry weight of shoots and root in the absence of pathogen. Comparing with the control sample, only the isolate T. harzianum in the interaction with the pathogen increased the plant height, wet and dry weight of shoots and root. According to study, in the greenhouse biological tests of biofungicide (Trichomax-HV) and Trichoderma spp. had shown good control on causal agent of the disease (Fusarium oxysporum f. sp. lycopersici). Among these treatments, T. harzianum had the best effect on the control of pathogenic fungi, which is due to the complex system of soil biology and ecology and the presence of antagonists in the rhizosphere and the power of root colonization and antagonist survival, soil pH and soil texture.
Conclusion: Based on the this study results, T. harzianum had the highest percentage of inhibition of pathogenic fungal growth as well as increase in height, fresh and dry weight of the plant and decrease in disease severity among the different treatments tested. In greenhouse conditions, T. harzianum prevented from tomato Fusarium wilt disease (caused by Fusarium oxysporum f. sp. lycopersici) in very large extent. Also this results showed that Trichomax-HV could be a viable alternative to chemical fungicides in controlling this disease.

Keywords

• Biological Control
• T. harzianum
• T. virens

20.1001.1.20084749.1400.35.3.2.1

Main Subjects

• Diseases

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References

1. Akrami M., and Ebrahimof A. 2010. The Investigation efficiency of combining two Trichoderma species in the biological control of Fusarium diseases of chickpea under greenhouse conditions. Journal Uni. Agricultural Science Tabriz 1(14): 75-83.
2. Amini K. 2009. Physiological race of Fusarium oxysporum sp. lycopersici in Kurdistan Province of Iran and reaction of some tomato cultivars to race 1 of pathogen. Plant Pathology 8: 68-73.
3. Banihashemi Z., and Dezeeuw D.J. 1969. Two improved methods for selectively isolating Fusarium oxysporum from soil and plant roots. Journal Plant Disease 53: 589-591.
4. Banihashmi Z. 2010. Reaction of Cucumis melo cultivars to races of Fusarium oxysporumsp. melonis the cause of melon vascular wit. Journal Plant Disease 46(1): 11-22.
5. Behboudi K., Sharifi Tehrani A., Hejaroud G.H., and ZAD J. 2005. Antagonistic effects of Trichoderma species on Phytophthora capsici, the causal agent of pepper root and crown rot. Plant Phytopathology 41: 345-362.
6. Booth C. 1971. The Genus Fusarium, Common wealth Mycological Institute Kew, Surrey, England, Printed in Great Britain by the Eastern Press Limited London and Reading 237 pp.
7. Chet I. 1987. Trichoderma– Application, mode of action and potential as a biocontrol agent of soil borne plant pathogenic fungi, Innovative Approaches to Plant Disease Control (I, Chet, Ed), J. Wiley and Sons, New York: 137-160.
8. Chet I., Inbar J., and Hadar Y. 1997. Fungal Antagonists and mycoparasites in: The mycota, Environmental and microbial relationships. Wicklow, D. T. And B. soders trom (eds). Vel 4. Springer- verlag, Berlin, Germany 165-184.
9. Cook R.J., and Baker K.F. 1983. The Nature and Practice of Biological Control of Plant Pathogens. American Phytopathological Society, 539 pp.
10. Davet P. 1979. Technique pour lanalyse des populations de Trichoderma et de Gliocladium virens dans le sol. Annuals Phytopathology 11: 529-533.
11. Dennis C., and Webester J. 1971. Antagonist properties of species group of Trichoderma Hyphal interaction. Transaction of the British Mycological Society 57: 363-369.
12. Gerlach W., and Nirenberg H. 1982. The Genus Fusarium a Pictorial atlas. Mitt. Biol. Bundesanst. Land-und Forstwirtsch. Berlin Dahlem, 406 pp.
13. Harman G. E., Howell C.R., Viterbo A., Chet I., and Lorito M. 2004. Trichoderma species- opportunistic, avirulent plant symbionts. Nature Reviews Microbiology 2: 43–56.
14. Howell C.R. 2003. Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Journal Plant Disease 87(1): 4-10.
15. Juber K.S., Hassan A.K., Alhamiri Y.N. 2014. Evaluation of biocontrol agents and chemical inducers for managing a vascular wilt of tomato caused by Fusarium oxysporum sp. lycopersici. Journal Biology Agriculture Healthcare 4(27): 335-343.
16. Kamal A.M., Elyousr A., and Mohamed H.M. 2009. Biological control of Fusarium wilt in tomato by plant Growth-promoting yeast and Rhizobacteria. Plant Pathology 25(2): 199-204.
17. Kari Dolatabadi H., and Mohammadi Goltapeh E. 2010. In vivo biological activity of Piriformospora indica, Sebacina vermifera and Trichoderma against Fusarium wilt of lentil. Journal of Plant Protection Reseach 2(2): 127-143.
18. Leslie J.F., and Summerell B.A. 2006. The Fusarium Laboratory Blackwell Publishing Professional, Ames, USA, 388pp.
19. Little T.M., and Hills F.J. 1978. Agricultural experimentation design and analysis. John Willy & Sons 350 pp.
20. Manafi R., Babai ahri A., Arznlo M., and Valizadeh M. 2012. Evaluation common resistance varieties a greenhouse tomato to Fusarium wilt in East Azerbaijan province and study of biological control of the disease. Journal of Sustainable Agricultural Production 22(1): 145-158.
21. Mohammadi N. 2010. Studies on pathogenicity and genetic diversity of some Iranian isolates Fusarium oxysporum sp lentis and determination of resistant lentil cultivars. M. SC. Thesis, Uni. Tarbiat Modares, Iran. 134p.
22. Nawrocka J., Malolepza V. 2013. Diversity in plant systemic resistance induced by Trichoderma. Biology Control 67(2): 149-156.
23. Nelson P.E., Toussoun T.A., and Marasas W.F.O. 1983. Fusarium Species: An Illustrated Manual for Identification. Pennsylvania State Uni. Press, Uni. Park. 193p.
24. Niknejad Kazempour M. 1992. Effect of several fungicides and Trichoderma fungi on tomato pathogen Fusarium wilt Fusarium oxysporumsp. lycopersici, MSc thesis, Faculty of Agriculture Tehran Uni. Karaj: 129 p.
25. Niknejad Kazempour M., and Sharifi Tehrani A. 1992. Effect of antagonistic fungi Trichoderma on pathogen Fusarium wilt of tomato Fusarium oxysporumsp. lycopersici. Laboratory and in greenhouse condition. Proc. 11^th Iran. Plant Protec. Cong. Uni. Gilan-Rasht: 165p.
26. Niknejad Kazempour M., Sharifi Tehrani A., and Okhovat M. 2000. Effect of Antagonistic fungi Trichoderma On the control of Fusarium wilt of tomato caused Fusarium oxysporum f.sp. lycopersici under greenhouse condition. Iranian Journal Agricultural Science 31(1): 31-37. (In Persian with English abstract)
27. Ojha S., Chatterjee NC. 2012. Induction of resistance in tomato against Fusarium oxysporum sp. lycopersici mediated through salicylic acid and Trichoderma harzianum. Journal Plant Protecttion Research 52(2): 220-225.
28. Panjekeh N., Saberian A., Afshari Azad H., and Salari M. 2011. Biological control of phomalingam, the causal agent of rapeseed blackleg, by Trichoderma and Bacillus subtilis Iranian Journal of Plant Pathology 47(1): 19-30. (In Persian with English abstract)
29. Papavizas G.C. 1985. Trichoderma and Gliocladium: Biology, Ecology, and Potential for Biocontrol. Annual Review of Phytopathology 23: 23-54.
30. Pourjam E., Kamali N., and Sahebani N. 2015. Elicitation of defense responses in tomato against Meloidogyne javanica and Fusarium oxysporum sp. lycopersici wilt complex. Journal Crop Protection 4(1): 29-38.
31. Rajik M., Biswas SK., and Shakti S. 2012. Biochemical basis of defense response in plant against Fusarium wilt through bio-agents as an inducer. Afr Journal Agriculture Research 7(43): 5849-5857.
32. Sajadi S.A., and Asemi H. 2008. Evaluation of biological control potential Trichoderma species against of tobacco collar rot in Mazandaran province; New Findings in Agriculture 2(3): 253-270.
33. Sivan A., Ucko O., and Chet I. 1986. Trichoderma harzianum and effective biocontrol agent of Fusarium Microbial Communities in Soil, 447pp.
34. Sundaramoorthy S., and Balabaskar P. 2013. Biocontrol efficacy of Trichoderma against wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici. Journal ApplBiol Biotechnology 1(3): 36- 40.
35. Walker J.C. 1981. Fusarium wilt of tomato. Monograph NO. 6, APS Press, 56 pp.
36. Zamanizadeh H.R., Hatami N., Aminaee M.M., and Rakhshandehroo F. 2011. Application of biofungicides in control of damping disease off in greenhouse crops as a possible substitute to synthetic fungicides. International Journal Environment Science Technology 8 (1): 129-136.