Evaluation of Reaction Thirty Dwarf Mahaleb Genotypes to Root and Crown Rot Phytophthora Disease using Laboratory Methods

Document Type : Research Article

Authors

• mohammad hajian ¹
• Ebrahim Gangi ¹
• Hamid Afzali ²

¹ Khorasan Razavi Agriculture and Natural Resource Research Center

² Razavi Agriculture and Natural Resource Research Center

10.22067/jpp.v30i4.54379

Abstract

Introduction: Iran is one of the biggest sweet cherry producers in the world. Turkey, the U.S.A and Iran are among the major producers of cherry in the world. Mahaleb(Prunus mahaleb L.) is used as a principal rootstocks for sweet and sour cherries in Iran. Mahaleb is a good rootstock on light, calcareous soils and arid climates in Iran. Since pathogenic species, P.citricola, P.cryptogea, P.dreschleri, P.cactorum, P. syringae, P.cinnammomi, P.megasperma, P.cambivora on mahaleb been reported by different researchers (Sajadinejad et al., 2011) According to the pathogenesis of four species of P. nicotianae, P.citricola, P.cactorum and P.citrophthora on stone fruit trees in Iran and other countries, based on the hypothesis that the relative resistance to Phytophthora species in selected dwarf Mahaleb genotypes adapted to environmental conditions exist and can be introduced as resistant rootstocks to crown rot disease, this study was conducted to evaluate the susceptibility of 30 selected dwarf Mahaleb genotypes to P.nicotianae, P.citricola, P.cactorum and P. citrophthora. Sofar, the selection of resistant or tolerant to Phytophthora cherry rootstock, studies have not been done. Therefore, it is appropriate to introduce the cherry trees for planting in various parts of the country, the need to evaluate several species of Phytophthora resistance or tolerance to the grades mentioned above. Considering the history of the pathogenesis off our species of P. nicotianae, P.citricola, P.cactorum and P.citrophthora the stone fruits this study aimed to evaluate the reaction of 30 cultivars of dwarf Mahaleb to P.nicotianae, P.citricola, P.cactorum, P.citrophthora was carried out.
Materials and Methods: In this study, the reaction of 30 dwarf Mahaleb genotypes were investigated to disease caused by four species of the fungus Phytophthora root and crown rot. Excised twig, annual and perennial shoots to measuring resistance using laboratory methods. Excised twig, excised annual and perennial shoots assessment based on Jeffers et al., (1981) and Matheron and Mircetich(1985) respectively.
Results and Discussion: In evaluating the reaction of excised twig, excised annual and perennial shoots dwarf mahaleb genotypes to four species of Phytophthora, rot and discoloration symptoms were observed, All control shoots of Mahaleb genotypes were evaluated in this trial did not show any symptoms of discoloration and Phytophthora rot but shoots treated with four species of Phytophthora showed that different degrees of light brown to dark discoloration and decay. The results of the analysis of variance showed that the genotypes of disease progression on the tissues of all three types of excised shoots inoculated with four species of Phytophthora were difference statistically significant. Also, the results of comparing the length average of progression of symptoms (necrosis) on this experiment were as follows which are outlined below.
P.cactorum
On the test assessment the reaction of excised twig, excised annual and perennial shoots dwarf mahaleb genotypes, least amount of sensitivity to species belongs to genotype199 with an average contamination of 12.44%, 162 with an average contamination of 1.42% and 162 with an average contamination 0.01 %, respectively.
P.citrophthora
On the test assessment the reaction of excised twig, excised annual and perennial shoots dwarf mahaleb genotypes, least amount of sensitivity to species belongs to genotype 265 with an average contamination of 15.67%, 155 and 162 with an average contamination of 0.01% and 262, 228, 224 and 188 with an average contamination 0.01 %, respectively.
P.citricola
On the test assessment the reaction of excised twig, excised annual and perennial shoots dwarf mahaleb genotypes, least amount of sensitivity to species belong to genotype 124 with an average contamination of 11.80%, 100 with an average contamination of 0.60% and 266 with an average contamination 3.13 %, respectively.
P.nicotianea
On the test assessment the reaction of excised twig, excised annual and perennial shoots dwarf mahaleb genotypes, least amount of sensitivity to species belong to genotype 120 with an average contamination of 16.15%, 100 and 95 with an average contamination of 0.01% and 188 with an average contamination 0.87%, respectively.
There were significant differences between the genotypes of disease progression in the tissues of all three types of excised shoots inoculated with four Phytophthora species.
Conclusion: The results showed that, some mahaleb genotypes (100, 124, 155, 162, 188, 195, 199, 224, 266 and 265) have potential resistance to species P.nicotianae, P.citricola, P.cactorum and P.citrophthora, that can be considered in breeding programs.

Keywords

• Crown
• Mahaleb
• Phytophthora
• Resistance
• Root rot

20.1001.1.20084749.1395.30.4.4.0

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References

1- Ahmadi K., 2015. Agricultural statistics. Horticultural Products. 3th ed, Ministry of Agricultural Jihade 147pp.

2- Bakhtiari M.H.V., and Khabaz H. 2010. Identification of Phytophthora Species Associated with stone fruits crown rot in Hamadan province. In: Proceeding of 19th Iranian Plant Protection Congress, 31 July3 August 2010.

3- Banihashemi Z., and Sartipi A. 2004. Identification of Phytophthora Species Associated with stone fruits crown rot in Fars province and reaction of certain root stock to P. cactorum. Science and Technology of Agricultural and Natural Resources, 8:241-249. (in Persian with English abstract)

4- Bielenin A., and Jones A.I. 1984. Prevalence and pathogenicity of Phytophthora spp. From Sour Cherry trees in 5-Michigan. Plant Disease, 72:433-476.

5- Elena K., and Tsipouridis K. 2000, Evaluation of resistance of stone fruit rootstocks to Phytophthora crown rot. Phytopathology, 148: 365-369

6- Erwin D.C., and Ribeiro O. K. 1996. Phytophthora Disease Worldwide. American Phytopathological Society Press, St. Paul, MN.

7- Exadaktylou E., and Thomidis T. 2005. Susceptibility of Gisela 5 and Maxma 14 cherry rootstocks to four Phytophthora species. Scientia Horticulturae, 106:125–128.

8- Hudson T., Hartman E.K., and Davies T.F. 1990. Plant Propagation Principles and Practices. 5th ed., Prentice Hall International Inc., New Jersey, USA.

9- Jeffers S.N., Aldwmckle H. S., Burr T. J., and Arneson P.A. 1981. Excised twig assay for the study of apple tree crown rot pathogens in vitro. Plant Disease, 65:823-825.

10- Matheron M.E., and Mircetich J.C. 1985. Seasonal variation in susceptibility of Juglans hindsii and paradox root stocks of english walnut trees to P. citricola. Phytopathology, 75: 970–972.

11- Mircetich M.S., and Matheron M.E. 1976. Phytophthora root and crown rot of cherry trees. Phytopathology, 66: 549–558.

12- Mozafarian V. 2005. Trees and shrubs of Iran. Published by Farhang Moaser. 1003pp.

13- Sajadinejad M., Ershad J., Mirabolfathi M., and Zamanizadeh H. 2011. Identification of Phytophthora species the causing root and crown rot of cherry trees in Tehran province. Iranian Journal of Plant Pathology, 46:81-88. (in Persian with English abstract)

14- Sharifi H., Bouzari N., and Keshavarz M. 2015. Evaluation of Relative Resistance in Five Stone Fruit Roots tocks to Phytophthora cactorum and P. drechsleri. Seed and Plant Improvement Journal, 31:307-323. (in Persian with English abstract)

15- Thomidis T. 2001.Testing variability in pathogenicity of Phytophthora cactorum, P. citrophthora and P. syringae to apple, pear, peach, cherry and plum rootstocks. Phytoparasitica, 29: 47–49.

16- Thomidis T., and Sotiropoulos T. 2003. Pathogenicity of 11 Phytophthora species on CAB-6P Cherry root stock. New Zealand Journal of Crop and Horticultural Science, 31:355–360.

17- Thomidis T., Karayiannis I., and Tsipouridis C. 2008.Suceptibility of thirty cherry genotypes on Phytophthora cactorum, P. citrophthora, P. citricola and P. parasitica. Phytopathology, 156: 446-451.

18- Thomidis T., Cullum J., Elena K., and Jeffers S.N. 2001. Relative resistance of four peach roots tocks to Phytophthora cactorum and P. megasperma. Phytopathology, 149:599-604.

19- Wilcox W.F., and Mircetich S.M. 1985. Pathogenicity and relative virulence of seven Phytophthora spp on Mahlaleb and Mazzard Cherry. Phytopathology, 75:221- 226.