Effect of Different Dormancy Breaking Treatments on Seed Germination of Salsifis (Teragopogon collinus) and Flixweed ( Descurainia Sophia) as Two Weed Species

Document Type : Research Article

Authors

• marzie mazhari ¹
• M. Tadayon ²

¹ Shaherkord University

² Shahrekord

10.22067/jpp.v30i3.30119

Abstract

Introduction: Weeds limit crops growth, development and yield through adjacency and competing and seed dormancy is a problem in controlling of weeds. Man has been always tried to optimize crop yield through weeds controlling. Weeds seed bank in the soil can affected weed management and their control methods. So identifying seed bank characters has important role in choice of management methods in seeds of many plant species which cannot germinate despite favorable environmental conditions. Main reasons for this problem, which is termed as seed dormancy, are hard and impermeable seed coat and presence of immature or dormant embryo. To break dormancy, a variety of methods are in vogue worldwide these days. Of all different methods of breaking dormancy, treatment of seed with certain chemicals including different types of plant growth regulators (PGRs) is contemplated as the most effective one. Gibberellic acid (GA3) is the most widely used PGR to improve seed germination in different plant species. Also, nitrate (such as KNO3) clearly stimulates the germination of dormant seeds. KNO3 is the most widely used chemical for promoting germination. Solutions of KNO3 are common in routine germination testing and are recommended by the Association of Official Seed Analysts and the International Seed Testing Association (ISTA) for germination tests of many species.Stratification refers to the method of placing seeds in layers alternating with layers of a moisture retaining medium, such as sand, peat or vermiculite, and keeping them at a cool temperature for a certain period, but it varies considerably from species to species. The combination of high moisture and low temperature appears to trigger off biochemical changes which transform complex food substances into simpler forms utilized by the embryo when it renews growth at germination. The use of the word “stratification” has recently been extended to include all forms of cold, moist treatment whether or not the seeds are placed in layers. Chilling plays an important role in providing the stimulus required to overcome dormancy, increase germination, and produce normal seedlings for Prunuspersica. Exogenous growth regulator treatments – gibberellins (usually gibberellic acid GA3 and GA4+7) and cytokinins (usually kinetin, benzyladenine) – have been shown to break dormancy in many seed species. The objective of the present study was to investigate effective methods in breaking the seed dormancy of Teragopogon collinus and Descurarina sophia and evaluate their response to different dormancy-breaking treatments, germination under different constant and alternating temperature, and using chemical, with most appropriate dormancy-breaking procedure for both species.
Materials and Methods: In order to evaluate the effect of different treatments on dormancy breaking and seed germination of Teragopogon collinus and Descurarina sophia an experiment was carried out as factorial based on a Randomized Complete Block Design (RCBD) with three replications at Shahrekord University in 2012. Treatments including different levels of gibberellic acid (0, 100, 250 and 500 mg) at two soaking time levels of 2 and 24 hour (along with scarification pre-treatment), concentrations of potassium nitrate (50, 100 and 150 mM) in 10 and 30 minutes (along with scarification pre-treatment), treatment of rotational temperature (5-15 and 10-20 °C), in full light, dark and rotational light periods (8 hr light and 16 hr dark) and mechanical treatments of dormancy breaking (application of boiling water).
Results and Discussion: Teragopogon collinus and Descurainia sophia had highest seed dormancy breaking under rotational temperature and light and application of gibberellic acid and potassium nitrate (along with scarification pre-treatment) had no effect on Teragopogon collinus and Descurarina sophia dormancy. According to the Teragopogon collinus and Descurainia sophia seeds dormancy breaking in mechanical treatments and the effect of rotational temperature and light, it can be concluded that the dormancy of Teragopogon collinus and Descurainia sophia seeds are intermittent. The results showed that the effect of temperature, time, and the interaction of light ×time of germination percent of Teragopogon collinus and Descurainia sophiawere were significant at 1%.The interaction between light and alternating temperature on Teragopogon collinus seed germination suggests that intermittent lighting conditions at alternating temperatures, increase the germination percentage of Teragopogon collinus. According to the Teragopogon collinus and Descurainia sophia seeds dormancy breaking in mechanical treatments and the effect of rotational temperature and light, it can be concluded that the dormancy of Teragopogon collinusan and Descurainia sophia seeds are intermittent.

Keywords

• Chemical treatments
• Rotational temperature and light
• Seeds
• Weed

20.1001.1.20084749.1395.30.3.18.2

---

References

1- Baskin C.C., and Baskin J.M. 1991. Nondeep complex morphophysiological dormancy in seed of Osmorhiza claytonii (Umbeliferae). American Journal of Botany: 78:588-593.

2- Baskin C.C., and Baskin J.M. 1998. Seeds: Ecology, Biogeography, Evolution of Dormancy and Germination. Academic Press, San Diego.

3- Baskin C.C., Milberg P., Andersson L., and Baskin J.M. 2001. Seed dormancy breaking and germination requirements of Drosera anglica an insectivorous species of the Northern Hemisphere. Acta Oecologica, 22: 1-8.

4- Baskin J.M., and Baskin C.C. 2004. A classification system for seed dormancy. Seed science Research, 14: 1-16.

5- Bradford K. J. 2002. Application of hydrothermal time to quantifying and modeling seed germination and dormancy Weed. Weed Science, 50:248-260.

6- Dissanayake P., George D.L., and Gupta M.L. 2010. Effect of light, gibberelic acid and abscisic acid on germination of guagule (Parthenium argentums Gray) seed. Industrial Crop and Production 32: 111-117.

7- Dogan Y.A. 2001. Syudy on the outecology of Reseda luteaL. (Resedaceae) distributed in western Anatolia. Turk. Journal Botanica, 25:137-148.

8- Gealy D.R., and Young F.L. 1985. Germination of may weed (Anthemis - cotula ) achenes and seed . Weed Science, 33: 69-73.

9- Jain R., and Singh M. 1989. Factors affecting goatweed (Scoparia dulcis) seed germination. Weed science, 37: 766-770.

10- Kallson L.M., and Milberg P. 2007. Seed dormancy pattern and germination preferences of the South African annual Papaver aculeatum. South African Journal of Botany, 73: 422-428.

11- Keshtkar A.R., Keshtkar H.R., Razavi S.M., and Dalfardi S. 2008. Methods to break seed dormancy of Astragalus cyclophyllon. African journal of Biotechnology, 7: 3847-3877.

12- Linding C.R. , and Lara-Carbera S. 2004. Effect of scarification and growing media on seed germination of crotalaria pamilalort. Seed Science and Technology, 32(1): 231-23.

13- Nadjafi M., Bannyan M., Tabrizi L., and Rastgoo M. 2006. Seed germination and dormancy breaking techniques for Ferula gammusa and Teucrium polium. Arid Environments, 64: 542-547.

14- Nandula V.K., Eubank T.W., Poston D.H., Koger C.H., and Reddy K.N. 2006. Factors affecting germination of horseweed (Conyza Canadensis). Weed Science, 54: 898-902.

15- Nishimoto R.K., and Mccarty L.B. 1997. Flactuating temperature and light influence seed germination of goosegrass (Eleusine indica). Weed science, 45: 426-429.

16- Rahnam-Ghahfarokhi A., and Tavakol-Afshari R. 2007. Methods for dormancy breaking and germination of Galbanum seeds (Ferula gummosa). Asian Journal. Plant science, 6(4): 611-616.

17- Zimdahl R.C. 2007. Fundamentals of Weed Science, Academic Pres.