Detection of Barley yellow mosaic virus by Serological and Molecular Methods in some Provinces of Iran

Document Type : Research Article

Authors

• S.A. Hosseini ¹
• B. Jafarpour
• Esmat Mahdikhani Moghadam ²
• mohsen mehrvar ²
• mohammad zaki aghl ²

¹ University of Birjand

² Ferdowsi University of Mashhad

10.22067/jpp.v28i4.45300

Abstract

In order to investigate the presence of Barley mild mosaic virus, 425 samples of barley and wheat from Razavi Khorasan, Northern Khorasan, Golestan, Yazd and Western Azarbayjan were collected in spring 2011. Plolyclonal antiserum was used in order to detect BaYMV by DAS-ELISA. The highest level of BaYMV infection was found in Azarbayjan gharbi and detect inRazavi Khorasan, Northern Khorasan and Golestan. Polymerase Chain Reaction (PCR) was carried out using specific primers for coat protein. A fragment of ~392 bp corresponding to BaMMV coat protein gene were amplified by PCR. Then one PCR product of Western Azarbayjan samples was sequenced and analyzed for additional confirmation. This is the first report of occurrence of BaMMV in aforementioned provinces of Iran.

Keywords

• PCR
• Barley mild mosaic virus
• Iran
• Coat protein

20.1001.1.20084749.1393.28.4.2.4

---

References

1- Adams M.J., and Jacquier C. 1994. Infection of cereals and grasses by isolates of Polymyxa graminis (Plasmodiophorales). Annual Applied Biology, 125:53-60.

2- Adams M.J. 2002. The mosaic viruses of winter barley: problemsand prospects. In: Proceedings of the BCPC Conference: Pestsand Diseases, 2002. Farnham, UK: British Crop Protection Council, 105–12.

3- Bauer E., Weyen J., Schiemann A., Graner A., Ordon F. 1997.Molecular mapping of novel resistance genes against barley mild mosaic virus (BaMMV). Theoretical and Applied Genetics, 95:1263–9.

4- Hariri D., Meyer M., Le Gouis J., Bahrman N., Fouchard M., Forget C., Andre A. 2000. Characterisation of BaYMV and BaMMV pathotypes in France. European Journal of Plant Pathology, 106: 365–72.

5- Hariri D., Meyer M., Prud’homme H. 2003. Characterization of a new Barley mild mosaic virus pathotype in France. European Journal of Plant Pathology, 109: 921–928

6- Huth W. 1989. Ein weiterer Stamm des Barley yellow mosaic virus (BaYMV) gefunden. Nachrichtenblatt des DeutschenPflanzenschutzdienstes, 41: 6–7.

7- Inouye T. and Saito Y. 1975. Barley yellow mosaic virus.CMI/AAB Description of Plant Viruses, 143.

8- Kashiwazaki S., Ogawa K., Usugi T., Omura T and Tsuchizaki T. 1989 Characterization of several strains of Barley yellow mosaic virus. Annals of Phytopathological Society of Japan, 55: 16–25.

9-Kashiwazaki S., and Hibino H. 1996. Genomic reassortmentof barley mild mosaic virus: Evidence for the involvementof RNA1 in pathogenicity. Journal of General Virology, 77: 581–585.

10- Kashiwazaki S. 1996. The complete nucleotide sequence and genome organization of barley mild mosaic virus (Na1 strain). Archives of virology, 141: 2077–2089.

11- Kanyuka K., Ward E., Adams M.J. 2003. Polymyxa graminis and the cereal viruses it transmits: a research challenge. Molecular Plant Pathology, 4: 393–406.

12- Kühne, T. 2009. Soil-borne viruses affecting cereals—Known for long but still a threat. Virus Research, 141:174–183.

13- Lee K.J., Kashiwazaki S., Hibi T., and So I.Y. 1996. Properties andcapsid protein gene sequence of a korean isolate of Barley mild mosaic virus. Annals of Phytopathological Society of Japan, 62:397–401.

14- Nomura K., Kashiwazaki S., Hibino H., Inoue T., Nakata E., Tsuzaki Y., Okuyama S. 1996. Biological and serological properties of two strains of Barley mild mosaic virus. Journal Phytopathol, 144: 103–107.

15- Okada Y., Kashiwazaki S., Kanatani R., Arai S., Ito K. 2003.Effects of Barley yellow mosaic disease resistant gene rym1on the infection by strains of Barley yellow mosaic virus and Barley mild mosaic virus. Theoretical and Applied Genetics, 106: 181–9.

16- Subr Z., Fomitcheva V.W., Kühne T. 2000. The complete sequence of RNA1 of the German isolate BaMMV-ASL1 and preparation of an antiserum specific to the nonstructural protein P3. Journal of Phytopathol, 148: 461–467.

17- Timpe U., Kühne T. 1994. The complete nucleotide sequence of RNA 2 of Barley mild mosaic virus (BaMMV). European Journal of Plant Pathology, 100: 233–242.

18- Usugi T. 1988. Epidemiology and management in Japan of Soil-borne cereal mosaic viruses with filamentous particles. In: Cooper JI, Asher MJC, eds. Developments in AppliedBiology II. Viruses with Fungal Vectors. Wellesbourne,UK: Association of Applied Biologists: 213–25.

19- Urcuqui-Inchima S., Haenni A.L., Bernardi F. 2001. Potyvirus proteins: a wealth of functions. Virus Research 74: 157-175.

20- Vaianopoulos C. 2008. Widespread occurance of Soil-born mosaic viruses in Belgium. In plant pathology, vol. Ph.D, pp.212. UCL, Louvain-la-Neuve, Belgium.

21- Vaianopoulos C., Legreve A., Moreau V., Steyer S., Maraite H., Bragard C. 2005..Occurrence of bymo- and furoviruses on wheat in Belgium. Parasitica, 6:47–53.

22- Werner K., Friedt W., Ordon F. 2000. Strategies for ‘pyramiding’resistance genes against the Barley yellow mosaic virus complex based on molecular markers and Dh-lines. In: Logue S,ed. Proceedings of the Eighth International Barley GeneticsSymposium, 2000. Adelaide, Australia: University of Adelaide, 200–2.

23- Zheng T., Cheng Y., Chen J.P., Antoniw J.F., and Adams M.J. 1999. The occurrence of barley mild mosaic virus (BaMMV)in China and the nucleotide sequence of its coat protein gene. Journal of Phytopathology, 147: 229-234.