The Effect of Cryphonectria parasitica Attack on Castanea sativa Histological Properties (Case Study: Visroud Forest- Guilan)

Document Type : Research Article

Authors

• Afrooz Hasani boosari ¹
• javad torkaman ²
• mehrdad ghodskhah daryaei ¹

¹ guilan university

² دانشگاه گیلان

10.22067/jpp.v31i4.36777

Abstract

Introduction: Chestnut (Castanea sativa) trees are generally distributed in the northern part of Iran. Chestnut trees provide eaten fruit and good-quality timber in many countries. Dried chestnut wood has a high resistance to decay due to extractive compounds, such as tannins. Chestnut wood is used for many applications, such as the construction of buildings and wooden furniture, shipbuilding, timbers, and musical instruments. This important tree species was used extensively until the occurence of the fatal disease chestnut blight (CBD) caused by Cryphonectria parasitica, which is a tree pathogen notable for the disease commonly known as chestnut blight, which primarily affects species of sweet chestnut including the American sweet chestnut (Castanea dentata) and European sweet chestnut (C. sativa). The pathogen has been referred to as the chestnut blight fungus. Castanea sativa has a ring-porous wood with larger early wood vessels formed in the spring. The aim of this study was to determine and compare the anatomy and the xylem vessels diameter and some ray attributes of diseased chestnut wood and healthy chestnut wood in the north of Iran.
Material and Methods: The sample tree was 20 m in height and 15-40 cm in diameter. The tests specimens were taken at 1.30 m height above the ground in the form of a disc that includes the infected area. In this study the effect of the Cryphonectria parasitica was investigated in wooden and bark samples from healthy and diseased trees in three diameter classes (small medium and large). Both diseased and healthy wood samples with dimensions of 2 x 2 x 2 cm were taken from a diseased chestnut tree to determine the anatomical features of thin (8-15 μm) transverse, radial, and tangential sections. This was done by using a microtome (Leica 820, Germany) for the light microscopical study of wood anatomy. All of the anatomical sections were prepared as recommended by Parsa pajouh and Schweingruber (1988). Then, some thin transversal section were prepared and after different stages of staining, microscope slides were prepared. To achieve statistically reliable results, the guidance provided by The International Association of Wood Anatomists Committee - IAWA Committee (1989) was used to determine the Tangential and Radial diameter of the Spring and summer vessels, and the width, height and number of rays in both healthy and diseased wood .To analysis of data used Anova and t-student tests and for compare mean Duncan test by Spss software.
Results and Discussion: Table 2 and 3 provide the average values of the radial and tangential diameter of the healthy and diseased wood vessels and frequency of the rays with their average of width and height. In both of the healthy and diseased parts of the chestnut wood, ring porosity was present, and the annual rings were distinct. The porosity and distinctness of the annual rings were not changed in the diseased wood. However, the tangential diameter of the vessels was 71.93 μm in the summer wood of the diseased part and 65.04 μm in the summer wood of the healthy part. These diameters for the spring wood were determined 457.25 μm and 337.21 μm, respectively. The tangential and radial diameters of the vessels were smaller in the healthy wood than in the diseased wood. The average vessel frequency in the springwood of the diseased and healthy parts were 6 and 6.2, respectively. The vessel frequency values in the summer wood were determined to be 44 and 29, respectively. The results showed that there was significant difference between summer vessel diameter in the three classes of diameter (large, medium and small diameter) at probability level of 5%, in diseased and healthy wood. Whereas the diameter of the radial and tangential spring vessels in diameter classes had no significant difference between medium and low diameter. The irregularities were observed in cross-section due to influence of fungi on structure of wooden fiber in chestnut, the fungi attacks wooden fiber more than other organs of trees. The irregular cell structure of the vessels and fibers of the diseased wood showed (Fig.5) some similarity with cancer in animals and humans.
Conclusion: This is the first detailed study that has been conducted on Cryphonectria parasitica diseased Iranian Chestnut trees. One way to prevent the spread of the disease in forests is to remove the diseased trees. These logs can be used in making furniture, shipbuilding, wooden building construction, and musical instruments. Heat treatment can be suggested for more dimensional stability and especially for sterilization of the timber obtained from the diseased trees.

Keywords

• Fiber
• Microtome
• Ray
• Summer and spring vessels

20.1001.1.20084749.1396.31.4.3.6

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References

1- Adamcikova K., and Juhasova G. 2002. Reproductive structures of Cryphonectria parasitica, Thaiszia Journal of Botany 12: 161-165.

2- Anagnostakis S.L. 1986. Diversity of Vegetative compatibility groups of Cryphonectria parasitica in Connecticut and Europe. Plant Disease, 70:536-538.

3- Anonymous. 2005. Cryphonectria parasitica. Bulletin Organisation Europeenne et Mediterraneenne pour la Protection des Plantes, 35: 271-2273.

4- Bisseger M., Rigling D., and Heiniger U. 1997. Population and disease development of Cryphonectria parasitica in European chestnut forests in the presence of natural hypovirulence, Phytopatology,87: 50-59

5- Bolvanský M., Ostrovský R., Kobza M., Kajaba P., Adamčikova K., Pažitný J., and Juhasova G. 2014. Spread of Chestnut Blight in Slovakia in Relation to the SiteTopography and Climatic Characteristics. Proc. IInd European Congress on Chestnut.Eds.: L. Radocz et al.Acta Hort. 1043, ISHS 2014.

6- Chaloux P. H. 2000. Virulence of Cryphonectria hypoviruses from previous release sites. MS Thesis, West Virginia University, Morgantown.

7- Gryzenhout M., Myburg H., Wingfield B. D., and Wingfield M. J. 2006. Cryphonectriaceae (Diaporthales), a new family including Cryphonectria , Chrysoporthe, Endothia and allied genera. Mycologia, 98: 239-249.

8- Gunduz G., Oral M.A., Akyuz M., Aydemir D., Yaman B., Asik N., Bulbul A.S., and Allahverdiyev S. 2016. Physical, Morphological properties and Raman Spectroscopy of Chestnut Blight Diseased Castanea Sativa Mill Wood. CERNE, V. 22 No.1 p. 43-58.

9- Hedayatii M.A., Mohajer M.R., Djazireie H., and Zobeiri M. 2003. An Investigation of Chestnut (Castanea sativa Mill.) Seedling Production in Gilan Province. Iranian Journal of Natural Resource.Vol.56, No.3:229-244. (In Persian with English summary)

10- Huang H., Norton J.D., Boyhan G.E., and Abrahams B. R. 1994. Graft compatibility among chestnut (Castanea) species. Hn: proceedings of the Annual Report of the Northern Nut Growers Assn: 140-148p.

11- Juhasova G., Admikova K., Ivanova H., and Kobza M. 2004. Situation of damage caused Cryphonectria parasitica to forest stands and orchards of Castanea sativa by 2001 in Slovakia, Journal of Horticultural Science, 3: 102-108.

12- Locci R. 2003. Chestnut blight: a epidemic checked by biological control frulian. Journal of Science, 4:27-45.

13- MacDonald W. L., and Fulbright D.W. 1991. Biological control of chestnut blight: Use and limitations of transmissible hypovirolance. Plant Disease, 75: 656-661.

14- Marra R. E., and Milgroom M.G. 2001. The mating system of the fungus Cryphonectria parasitica selfing and self-incompatibility,Heredity,86:134-143

15- Mehdinejad Sh., Khodaparast S.A., and Jamali S. 2009. Fertility status and mating type distribution of Cryphonectria parasitica causal agent of Chestnut blight in Guilan province. Iranian Journal of Plant protection Science, 40(1) 85-92. (In persian with English summary)

16- Newbanks D., Bosch A., and Zimmermann M.H., and Neth J. 1983. Plant Pathology, 76, 196.

17- Parsa pajouh D., and Schweingruber F.H. 1988. Atlas of Iranian north woods. Tehran University Publication, pp: 136.

18- Qezi E., Khodaparast S.A., and Niknejad M. 2009. Study on the Morphological and Virulence variability of Cryphonecteria parasitica causal agent of chestnut blight in Guilan province, Iranian Journal of Plant Pathology, 45 (1): 25-35. (In Persian with English summary)

19- Sander I. L. 1974. Castanea, Chestnut. In: Schopmeyer CS, Tech. coord. A forest servi Seeds of woody plants in the United States. Agriculture Hand book.450. Washington, DC: USDA forest service: 273-275p.