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مائده شهیری طبرستانی کامران رهنما محسن جهانشاهی سعید نصرالله نژاد محمدحسین فاطمی

چکیده

فعالیت ضد¬قارچی جدایه Trichoderma atroviridae (6022) در¬برابر قارچ¬های بیمارگر خاکزاد Macrophomina phaseolina و Sclerotinia sclerotiorum درشرایط آزمایشگاه، مورد بررسی قرار گرفت. در آزمون کشت متقابل، جدایه آنتاگونیست به سرعت، رشد قارچ¬های بیمارگر را متوقف نمود. متابولیت¬های فرار جدایه آنتاگونیست در کشت همزمان، 24، 48 و 72 ساعته در برابر قارچ¬های بیمارگر، اثرات بازدارندگی چشمگیری نشان دادند که با گذشت زمان و مسن تر شدن پرگنه قارچ تریکودرما، میزان تولید این متابولیت¬ها افزایش و در¬ نتیجه اثرات بازدارندگی بیشتری مشاهده شد. غلظت¬های مختلف متابولیت¬های غیرفرار استخراج شده از محیط غذایی مایع و توده قارچی، توانستند به¬خوبی رشد میسیلیومی و تولید اسکلروت قارچ¬های بیمارگر را کنترل نمایند. متابولیت¬های غیرفرار استخراجی از توده قارچی، بازدارندگی بهتری نشان دادند و توانستند در غلظت 15 درصد به ترتیب تا 62/87 و 100 درصد از رشد M. phaseolina و S. sclerotiorum جلوگیری نمایند. از طریق چهار فرایند ساده آزمایشگاهی جهت استخراج (با حلال¬های آلی ان-هگزان و متانول؛ تکنیک Headspace و سوکسله توده قارچی) و با استفاده از دستگاه گاز کروماتوگرافی جرمی، 61 ترکیب متابولیت ثانویه شناسایی شدند که بیشتر آن¬ها از گروه آلکان¬ها بودند. کلیه ترپنوئیدهای شناسایی شده در این بررسی، فقط در تکنیک Headspace مشاهده شدند. از ترکیبات ضد¬قارچی شناسایی شده در این جدایه، 1-¬پنتانول، ایزوآمیل الکل و 2-اتیل-1-¬هگزانول؛ پالمیتیک اسید و استیریک اسید؛ بیس(2- اتیل ¬هگزیل) فتالات و دی-¬ان¬اکتیل فتالات می¬باشند. وجود این ترکیبات، اثرات ضد¬قارچی متابولیت ثانویه را توجیه
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جزئیات مقاله

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ارجاع به مقاله
شهیری طبرستانیم., رهنماک., جهانشاهیم., نصرالله نژادس., & فاطمیم. (2016). استخراج و شناسایی متابولیت¬های ثانویهTrichoderma atroviridae (6022) و بررسی اثرات ضدقارچی آنها. مطالعات حفاظت گیاهان, 31(1), 131-141. https://doi.org/10.22067/jpp.v31i1.55861
نوع مقاله
علمی - پژوهشی