ترجیح غذایی کنه شکارگر Amblyseius swirskii روی سفید بالک گلخانه Trialeurodes vaporariorumتیمار شده و تیمار نشده با قارچ Beauveria bassiana

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه تهران

چکیده

کنه شکارگر Amblyseius swirskii و قارچ بیمارگرBeauveria bassiana از دشمنان طبیعی مهم سفید بالک گلخانه Trialeurodes vaporariorum هستند. در بررسی کارایی و انتخاب دشمنان طبیعی، تعیین ترجیح غذایی آنها، هنگامی که دشمن طبیعی در برابر میزبان آلوده یا غیر آلوده به عوامل بیمارگر قرار می‌گیرد، از اهمیت خاصی برخوردار است. در این تحقیق ترجیح غذایی کنه شکارگر دو روز پس از بالغ شدن در برخورد با پوره سن سوم سفید بالک گلخانه غیر آلوده و تیمار شده با قارچ B. bassiana در دو تیمار زمانی 24 و 48 ساعت بعد از آلودگی شکار با استفاده از شاخص بتای منلی تعیین شد. شکارگرهایی که 24 ساعت گرسنگی داده شده بودند بر روی دیسک‌های برگی خیار رهاسازی شدند و پس از 24 ساعت تعداد سفید‌بالک‌های مصرف شده شمارش شدند. آزمایش درون ظروف پتری تهویه‌دار و به مدت 24 ساعت انجام شد. براساس نتایج مقدار شاخص در بازه زمانی 24 و بازه زمانی 48 ساعت پس از تیمار شدن میزبان به قارچ، به ترتیب 19/۰±15/4 و 12/۰±23/۲ برآورد شد. نتایج نشان داد، A. swirskii در تغذیه از سفید بالک‌های غیر آلوده در مقابل سفید بالک‌های تیمار شده با قارچ در بازه زمانی 24 ساعت (0034/0>P) و 48 ساعت (0001/0P

کلیدواژه‌ها


عنوان مقاله [English]

Prey Preference of Predatory mite Amblyseius swirskii (Athias-Henriot) (Acari: Phytoseiidae) on untreated and Beauveria bassiana-treated of Trialeurodes vaporariorum (Homoptera: Aleyrodidae

نویسندگان [English]

  • Marjan Seiedy
  • Mahdi Tork
University of Tehran
چکیده [English]

Introduction
The predator Amblyseius swirskii and entomopathogenic fungus Beauveria bassiana are important biocontrol agents of Trialeurodes vaporariorum. Determination of the host preference of predators in the fields when receiving signals related to either untreated and Beauveria bassiana-treated pest is important.
Materials and Methods
In this research, the prey preference of adult female (2 days old) of A. swirskii was determined on untreated and Beauveria bassiana-treated of Trialeurodes vaporariorum (Homoptera: Aleyrodidae) in various treatments base on Manly Index. These different treatments consisted of two time intervals; 24 and 48 h post-inoculation of greenhouse whiteflies with 1×105 conidia /ml of B. bassiana with 13 replicates. 24-h starved predators were added to the leaf discs singly then the number of consumed untreated and B. bassiana-treated T. vaporariorum in each Petri dish was assessed after 24 h.
Results and Discussion
The results revealed that mean number of consumed B. bassiana-treated T. vaporariorum in two time intervals; 24 and 48 h. after inoculation were 4/15±0/19 and 2.23±0/12, respectively. This investigation showed a significant preference of A. swirskii towards untreated T. vaporariorum in 24 (P< 0.0034) and 48 h. (P

کلیدواژه‌ها [English]

  • Amblyseius swirskii
  • Entomopathogen
  • Greenhouse Whitefly
  • prey preference
1- Aroiee H., Mosapoor S., and Karimzadeh H. 2005. Control of greenhouse whitefly (Trialeurodes vaporariorum) by thyme and peppermint kmitl. KMITL Science Journal, 5:511-514.
2- Briggs C.J. 1993. Competition among parasitoid species on a stage-structured host and its effect on host suppression. American Naturalist, 141: 372-397.
3- Bruck D.J., and Lewis L.C. 2002. Carpophilus freemani (Coleoptera: Nitidulidae) as a vector of Beauveria bassiana. Journal of Invertebrate Pathology, 80: 188-190.
4- Byrne D.N., and Bellows T.S. 1991. Whitefly Biology. Annual Review of Entomology, 36: 431-457.
5- Carey J.R. 1993. Applied Demography for Biologists with Special Emphasis on insect. Oxford University Press, New York. 206 pp.
6- Choi W.I., Lee E.H., Choi B.R., Park H.M., and Ahn Y.J. 2003. Toxicity of plant essential oils to Trialeurodes vaporariorum (Homoptera: Aleyrodidae). Journal of Economic Entomology, 96: 1479–1484.
7- Chouvenc T., Su N.Y., and Elliott M.L. 2008. Interaction between the subterranean termite Reticulitermes flavipes (Isoptera: Rhinotermitidae) and the entomopathogenic fungus Metarhizium anisopliae in foraging arenas. Journal of Economic Entomology, 101: 885-893.
8- Colfer R.G., and Rosenheim J.A. 1995. Intra-guild predation by coccinellid beetles on an aphid parasitoid, Lysiphlebus testaceipes. Proceeding of the Beltwide Cotton Conference. pp.1033-1036.
9- Cory J.S., and Hoover K. 2006. Plant-mediated effects in insectpathogen interactions. Trends in Ecology & Evolution, 21:278-286.
10- Cranshaw W.S. 2007. Greenhouse Whitefly. Colorado State University Extension. http://www.ext.colostate.edu/pubs/insect/05587.html.
11- Down R.E., Guthbertson A.G.S., Mathers J.J., and Walters K.F.A. 2009. Dissemination of the entopathogenic fungi, Lecanicillum longisporum and L. muscarium, by the predatory bug, Orius laevigatus, to provide concurrent control of Myzus persicae, Frankliniella occidentalis and Bemisia tabaci. Biological Control, 50: 172-178.
12- Dunkel F.V., and Jaronski S.T. 2003. Development of a bioassay system for the predator Xylocoris flavipes (Reuter) and its use in subchronic toxicity/pathogenicity studies of Beauveria bassiana (Bals.) Vuill. Strain GHA. Journal of Economic Entomology, 96: 1045-1053.
13- Evans E.W., and England S. 1996. Indirect interaction in biological control of insects: pests and natural enemies in alfalfa. Ecological Applications, 6: 920-930.
14- Goettel M. S., Poprawski T.J., Vandenberg J.D., Li Z., and Roberts D.W. 1990. Safety to nontarget invertebrates of fungal biocontrol agents. In: Laird, M., Lacey, L. A., and Davidson, F. W. (Eds.), Safety of Microbial Insecticides. CRC Press, Florida, pp. 209-231.
15- Hamdi F., Fargues J., Ridray G., Jeannequin B., and Bonato O. 2011. Compatibility among entomopathogenic hyphocreales and two beneficial insects used to control Trialeurodes vaporariorum (Hemiptera: Aleurodidae) in Mediterranean greenhouses. Journal of Invertebrate Pathology, 108:22–29.
16- James R.R., and Lighthart B. 1994. Susceptibility of the convergent ladybeetle (Coleoptera: Coccinellidae) to four entomogenous fungi. Environmental Entomology, 23: 190-192.
17- Kepler R. M., and Bruck D. J. 2006. Examination of the interaction between the Black Vine Weevil (Coleoptera: Curculionidae) and an entomopathogenic fungus reveals a new tritrophic interaction. Environmental Entomology, 35: 1021-1029.
18- Magalhaes B.P., Lord J.C., Wraight S.P., Daoust R.A., and Roberts D.W. 1988. Pathogencity of Beauveria bassiana and Zoophthor aradicans to the coccinellid predators Coleomegilla maculate and Eriopsis connexa. Journal of Invertebrate Pathology, 52: 471-473.
19- Malekan N., Hatami B., Ebadi R., Akhavan A., and Radjabi R. 2015. Evaluation of entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium on different nymphal stages of greenhouse whitefly Trialeurodes vaporariorum in greenhouse conditions. Biharean Biologist, 9 (2): 108-112.
20- Manly B.F.J. 1974. A model for certain types of selection experiments. Biometrics, 30: 281-294.
21- Meyling N.V., Pell J.K., and Eilenberg J. 2006. Dispersal of Beauveria bassiana by the activity of nettle insects. Journal of Invertebrate Pathology, 93:121-126.
22- Pell J.K., Pluck R., Clark S.J., Kenward M.G., and Alderson P.G. 1997. Interactions between two natural enemies, the entomopathogenic fungus Erynia neoaphids Remaudiere and Hennebert (Zygomycetes: Entomophthorales) and the predatory beetle Coccinella septempunctata L. (Col., Coccinellidae). Journal of Invertebrate Pathology, 69: 261-268.
23- Pell J.K., and Vandenberg J.D. 2002. Interaction among aphid Diuraphis noxia, the entomopatogenic fungus Paecilomyces fumosoroseus and the coccinellid Hippodamia convergens. Biocontrol Science & Technology, 12: 217-224.
24- Poprawski T.J., Greenberg S.M., and Ciomperlik M.A. 2000. Effect of host plant on Beauveria bassiana and Paecilomyces fumosoroseus induced mortality of Trialeurodes vaporariorum (Hom., Aleyrodidae). Biological control, 29: 1048-1053.
25- Roy H.E., and Pell J.K. 2000. Interactions between entomopathogenic fungi and other natural enemies: implications for biological control. Biocontrol Science & Technology, 10: 737-752.
26- Roy H., Pell J.K., Clark S.J., and Alderson P.G. 1998. Implication of predator foraging on aphid pathogen dynamics. Journal of Invertebrate Pathology, 71: 236-247.
27- Roy H.E., Pell J.K., Clark S.J., and Alderson P.G. 2001. Targeted dispersal of the aphid pathogenic fungus Erynia neoaphidis by aphid predator Coccinella septempunctata. Biocontrol Science & Technology, 11: 99-110.
28- Seiedy M. 2014. Feeding preference of Phytoseiulus persimilis Athias-Henriot (Acari: Phytosei-idae) towards untreated and Beauveria bassiana treated Tetranychus urticae (Acari: Tetranychidae) on cucumber leaves. Persian Journal of Acarology, 3: 91–97.
29- Seiedy M., Tork M., and Deyhim F. 2015. Effect of the entomopathogenic fungus Beauveria bassiana on the predatory mite Amblyseius swirskii (Athias-Henriot) (Acari: Phytoseiidae) as a non-target organism. Systematic and Applied Acarology, 20: 241–250.
30- Seiedy M., Saboori A., Allahyari H., Talaei-Hassanloui R., and Tork M. 2012. Functional response of Phytoseiulus persimilis (Acari: Phytoseiidae) on untreated and Beauveria bassiana-treated adults of Tetranychus urticae (Acari: Tetranychidae). Journal of Insect Behavior, 25: 543–553.
31- Seiedy M., Saboori A., and Zahedi-Golpayegani A. 2013. Olfactory response of Phytoseiulus persimilis (Acari: Phytoseiidae) to untreated and Beauveria bassiana-treated adults of Tetranychus urticae (Acari: Tetranychidae) on cucumber plants. Experimental and Applied Acarology, 60: 219–227.
32- Sengonca C., and Frings B. 1985. Interference and competitive behavior of the aphid predators, Chrysoperla carnea and Coccinella septempunctata in the laborarory. Entomophaga, 30: 245-251.
33- Van Lenteren J.C., and Noldus L.P.J.J. 1990. Whitefly-plant relationships: behavioral and ecological aspects. In: Gerling, D. (Ed.), Whiteflies: Their Bionomics, Pest Status and Management. Intercept, Andover, UK, pp. 187–210.
34- Van Lenteren J.C., and Woets J. 1988. Biological and integrated pest control in greenhouses. Annual Review of Entomology, 33: 239–269.
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