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اعظم سلیمانی عصمت مهدیخانی مقدم حمید روحانی

چکیده

در این تحقیق فعالیت آنزیمی لیپاز و کیتیناز قارچ­های همراه نماتد سیستی چغندرقند (Heterodera schachtii) جمع­آوری شده از استان خراسان رضوی مورد مطالعه قرار گرفت. به این منظور در سال زراعی 95-94، حدود 300 جدایه قارچی از سیست، ماده و تخم و لارو نماتد سیستی چغندرقند جداسازی گردید. جدایه­های مذکور پس از خالص­سازی، مورد بررسی­های ماکروسکوپی، میکروسکوپی و مولکولی قرار گرفتند و با استفاده از کلیدهای معتبر شناسایی شدند. از میان آن­ها شش گونه شامل Simplicillium lanosoniveum، Penicillium chrysogenum، Colletotrichum gloeosporioides، Neonectria macrodidyma، Fusarium oxysporum و Alternaria chlamydospora شناسایی شدند. در بررسی نسبی فعالیت آنزیم لیپاز، قارچ C .gloeosporioides بیشترین میزان تولید این آنزیم را نشان داد و در بررسی نسبی فعالیت کیتیناز، بیشترین تولید این آنزیم مربوط به قارچ P .chrysogenum بود.

جزئیات مقاله

کلمات کلیدی

چغندر قند, نماتد سیستی, قارچ, لیپاز, کیتیناز

مراجع
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ارجاع به مقاله
سلیمانیا., مهدیخانی مقدمع., & روحانیح. (2020). بررسی نسبی تولید آنزیم لیپاز و کیتیناز قارچ¬های جدا شده از نماتد سیستی چغندرقند در شرایط آزمایشگاهی. مطالعات حفاظت گیاهان, 34(1), 1-13. https://doi.org/10.22067/jpp.v34i1.63886
نوع مقاله
علمی - پژوهشی