شناسایی نماتدهای انگل گیاهی مزارع پنبه در استان خراسان جنوبی

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه فردوسی مشهد

چکیده

به منظور شناسایی نماتدهای انگل گیاهی مزارع پنبه در استان خراسان جنوبی، در سال های 1392 و 1393، تعداد 56 نمونه خاک از مناطق مختلف استان جمع آوری گردید. شستشوی خاک و استخراج نماتدها با استفاده از روش تلفیق الک و سانتریفیوژ جن کینز (1964) و سینی وایت هد (1965) و تثبیت و انتقال آن ها به گلیسرین طبق روش دگریس (1969) انجام گرفت. سپس از نماتدهای استخراج شده به تفکیک جنس، اسلایدهای میکروسکوپی تهیه و با استفاده از میکروسکوپ نوری مورد مطالعه قرار گرفت. پس از بررسی های میکروسکوپی، اندازه گیری های لازم و رسم تصاویر مورد نیاز، شناسایی گونه ها با استفاده از منابع و کلیدهای معتبر انجام گرفت و تعداد 15 گونه نماتد متعلق به 10 جنس شناسایی گردید که عبارتند از:
Aphelenchus avenae, Basiria graminophila, Boleodorus clavicaudatus, B. pakistanensis, B. thylactus, Ditylenchus hexaglyphus, D. tenuidens, D. valveus, Geocenamus rugosus, Filenchus vulgaris, Pratylenchus thornei, P. neglectus, Scutylenchus quadrifer, Merlinius brevidens,Zygotylenchus guevarai.
از بین جنس و گونه های شناسایی شده، سه گونه Boleodorus pakistanensis، Ditylenchus hexaglyphus و D. valveus برای اولین بار از ایران گزارش و توصیف می شوند.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of Plant-parasitic Nematodes in Cotton Fields in Southern Khorasan Province

نویسندگان [English]

  • A. Bakhshani
  • E. Mahdikhani-Moghadam
  • S. Baghaee Ravari
Ferdowsi University of Mashhad
چکیده [English]

Introduction: Gossypium species are distributed in the arid to semiarid regions of the tropical and subtropical, generally shrubs or shrub-like plants. The leaves are broad and lobed with three to five lobes. The seeds are contained in a capsule called a "boll", each seed surrounded by fibers. Commercial species of cotton plant are Gossypium hirsutum (>90% of world production), G. barbadense (3-4%), G. arboreum and G. herbaceum (together, 2%). Cotton is grown as a cash crop; it is often grown in a monoculture system that favors the development of a nematode community dominated by one or a few parasitic species. The different genera of plant parasitic nematodes such as Haplolaimus, Helicotylenchus, Blanolaimus, Pratylenchus, Paratrichodorus, Meloidogyne, Merlinius, Xiphinema, Tylenchorynchus, Rotylenchulus, Scutellonema, Heterodera, Ditylenchus, Longidorus, Aphelenchoides, Aphelenchus were recorded from cotton fields in the world. The objective of this study was to investigate the plant parasitic nematodes associated with cotton fields in Southern Khorasan province of Iran.
Materials and Methods: In order to identify the plant parasitic nematodes in cotton fields in Southern Khorasan province, 56 soil samples were collected from different areas during 2013 and 2014. Soil samples were washed and nematodes were extracted by combined sieving and centrifugal-flotation method of Jenkins (1964) and Whitehead tray method (1965). Nematodes were fixed and transferred to glycerin by using the method of De Grisse (1969). The permanent slides were prepared and the nematodes were studied by light microscope.
Results and Discussion: In this study, 15 species from 10 genera related to order Tylenchomorpha, were identified as follows: Aphelenchus avenae, Basiria graminophila, Boleodorus clavicaudatus, B. pakistanensis, B. thylactus, Ditylenchus hexaglyphus, D. tenuidens, D. valveus, Geocenamus rugosus, Filenchus vulgaris, Pratylenchus neglectus, P. thornei, Scutylenchus quadrifer, Merlinius brevidens, Zygotylenchus guevarai. One genus of cotton-parasitic nematodes known to cause yield loss were found in this survey (Pratylenchus spp). Other parasitic nematodes not known to cause yield loss also were found. Three species, including Boleodorus pakistanensis, Ditylenchus hexaglyphus, D. valveus are reported as new records for Iran. Boleodorus pakistanensis is characterized by four incisures, head low, unstriated and conoid shape, stylet 8.5-10µm with cone short and slender knobs, non-muscular median bulb, Basal bulb pyriform, posterior vulva (v=68.1-72.4 ; v/=77.5-88.4). Tail elongate-conoid, central arcuate, ending in a finely rounded terminus. B. pakistanensis resembles, B. flexuosus B. teres, B. thylactus and B. cylindricus. B. thylactus but can be differentiated by the more posterior position of the opening of the dorsal gland (2 vs 3-5); B. flexuosus has a more anterior vulva (V=54-63 vs 68.1-72.4); B. teres has a more anterior vulva (V=54-63 vs 68.1-72.4), tail shape and stylet length (10-12 vs 8.5-10); B. cylindricus has a more anterior position of the opening of the dorsal gland (7 vs 3-5) and more tail (88-101 vs 72-88.5). Ditylenchus hexaglyphus is characterized by six lines in lateral fields, low head, stylet 8-9µm with cone shorter than the shaft and small elongated knobs, non-muscular median bulb, Posterior bulb offset, posterior vulva (v=82.3 -83.5; v/=87.8-89.6). Tail ventrally curved, terminus rounded. Having non-muscular median bulb, six incisures, short stylet and posterior vulva. D. hexaglyphus resembles D .medians, D. taylori, D. affinis, and D. tuberosus. However, none of these species have such small stylet knobs, and all but D. affinis have longer PUS than that observed in D. hexaglyphus. D. taylori more anterior vulva (V=75-77 vs 82.3-83.5) and tail appears to be thinner (c/=6-7 vs 2.9-3.8); D. affinis has a more anterior vulva (V=76-80 vs 82.3-83.5). Ditylenchus valveus is characterized by six incisures, annulated head, stylet 8-9µm with short cone and small rounded knobs, muscular median bulb with small. Posterior bulb offset, posterior vulva (v=74.3 -81.5; v/=84.9-89.2). Tail terminus rounded. Because of six incisures, short stylet, vulva position, oesophagus structure and tail shape, D. valveus resembles D. acutatus D. myceliophagus and D. medicaginis. D. myceliophagus differs from D.valveus by tail shape and generally overlapping oesophagus; D. medicaginis differs from D. valveus mainly by tail shape and also slightly more posterior vulva position (78-83 vs 74.3-81.5); D. acutatus differs from D. valveus mainly by tail shape.
Conclusion: In this study, 15 species from 10 genera of plant parasitic nematodes were identified. Among these genera and species, three species including Boleodorus pakistanensis, Ditylenchus hexaglyphus, D. valveus are reported as new records for Iran.

کلیدواژه‌ها [English]

  • Boleodorus pakistanensis
  • Ditylenchus hexaglyphus
  • D. valveus
  • nematode
  • taxonomy
1- Akbarloo H., Nemati N., and Khtibi Y. 2014. Cultivation cotton. Tehran: Publishing company's textbooks. 145pp.
2- Barooti SH., and Alavi A. 2002. Plant Nematology. Application of Agricultural Sciences Publications. 278pp.
3- Bakhshani A., Mahdikhani-Moghadam E., Baghaee-Ravari S., Alvani S., and Abdzadeh J. 2014. Identification of plant parasitic nematodes in cotton fields in South Khorasan province. National Conference on Advances in Agricultural Issues, Tehran, Iran.
4- Bakhshani A., Mahdikhani-Moghadam E., and Baghaee-Ravari S. 2014. Ditylenchus valveus species reported from Iran. Proceedings of 21th Iranian Plant Protection Congress. Uremia, Iran, pp. 327. (in Persian with English abstract).
5- Brzeski M. W. 1991. Review of the genus Ditylenchus Filipjev, 1936, (Nematoda: Anguinidae). Revue de Nematology, 14 : 9-59.
6- De Grisse A. T. 1969. Redescriptionou modification de quelques techniques utiliseesdans L etude desnematodes phytoparasitaires. MededRijksfaculteit der landbouwetenschappen Gent, 34: 351-369.
7- De Ley P., and Blaxter M. L. 2002. Systematic position and phylogeny. In: D. L. Lee (Ed.) The Biology of Nematodes, London, Taylor and Francis:1-30.
8- Esmaili M., Heidari R., and Golhasan B. 2014. Introduction of several species of Ditylenchus (Nematoda: Anguinidae) of Kermanshah Province. Proceedings of 21th Iranian Plant Protection Congress. Uremia, Iran, pp. 3 31. (in Persian with English abstract).
9- Ghanbarnia K., Kheiri A., and Poorjam A. 1998. Identification of plant parasitic nematodes in cotton fields of Gorgan-Gonbad and Varamin using their morphologic and morphometric. Proceedings of 13th Iranian Plant Protection Congress. Karaj, Iran, pp. 115. (in Persian with English abstract).
10- Geraert E. 2008. Tylenchidae of the world. Academic press, 540 pp.
11- Harati Z., Vafaee M., and Khosroshahi M. 2012. Cytological and morphological study hybrids resulting from crosses between diploid cotton species native species Abbas and Arya with Aroreum species (Gossypium arboretum). Plant Biology, 3(8): 77- 90.
12- Hilloks R. J. 1992. Cotton Diseases. CAB press. 740 pp.
13- Jenkins W. R. 1964. A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Disease Reporter, 48: 692.
14- Kardovan Ghabel V., Bagherie Najarie M. B., Alishah A., and Soltanloo H. 2013. Correlation analysis of agronomic traits, quality fabrics and AFLP molecular markers in hybrid cotton (Gossypium hirsutum, Gossypium barbadens). Journal Modified Crops, 5(12): 63-74.
15- Krussberg L. F., and Sasser J. N. 1956. Host- parasite relationship of the lance nematode in cotton roots. Phytopathology, 46: 505-510.
16- Khan A. M., and Siddiqi M. R. 1968. Three new species of Nothotylenchus (Nematoda: Neotylenchidae), from North India. Nematologica, 14: 369-376.
17- Miraeez A., Heidari R., Tanhamaafi Z., and Aghighi M.R. 2014. A new report from the three species of the genus Ditylenchus (Anguinidae: Nematoda) from Iran. Proceedings of 21th Iranian Plant Protection Congress. Uremia, Iran, pp. 321. (in Persian with English abstract).
18- Siddiqi M. R. 1963. Boleodorus pakistanensis. sp.n. (Nematoda: Tylenchida), found associated with pine roots in Abbottabad, Pakistan. Science Culture, 29: 562-563.
19- Whitehead A. G., and Hemming J. R. 1965. A comparison of some quantitative methods of extracting vermiform nematodes from soil. Annual Applied Biology, 55: 25-38.
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