شناسایی مولکولی میزبان‌های علفی ویروس پیچیدگی برگ زرد گوجه‌فرنگی در جنوب استان کرمان

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه جیرفت

2 دانشگاه بیرجند

3 شهید باهنر کرمان

چکیده

ویروس پیچیدگی برگ زرد گوجه فرنگی (Tomato yellow leaf curl virus, TYLCV) در سال‌های اخیر خسارت گسترده‌ای به کشت‌های گوجه فرنگی و انواع کدوئیان در مناطق جنوب و جنوب شرقی ایران وارد نموده است. به منظور شناسایی علف‌های هرز میزبان این ویروس، از علف‌های هرز حاشیه و داخل گلخانه‌ها و مزارع به شدت آلوده انواع کدوئیان و گوجه فرنگی در مناطق منوجان، کهنوج، فاریاب، عنبرآباد و جیرفت نمونه‌برداری صورت گرفت. نتایج به‌دست آمده نشان داد که علف‌های هرز ارزق اورشلیمی (Chrozophora tinctoria)، آفتاب پرست (Heliotropium annum)، پنیرک (Malva neglecta) و سلمه تره (Chenopodium murale) به این ویروس آلوده بودند. به منظور مقایسه جدایه‌های TYLCV موجود در علف‌های هرز آلوده، قطعه 550 جفت بازی مربوط به بخشی از ناحیه بین ژنی و بخشی از ژن پروتئین پوششی 4 جدایه مختلف ویروس تعیین ترادف گردید. بررسی شباهت ژنتیکی این جدایه‌ها با جدایه‌های موجود در بانک جهانی ژن نشان داد که این جدایه‌ها با جدایه‌های مورد مقایسه از بانک ژن در گستره 98/99-24/93 درصد در سطح نوکلئوتیدی و 15/98-42/87 درصد در سطح آمینواسیدی تشابه دارند و تعداد 66 جهش در بین توالی‌های مورد مقایسه در این تحقیق در سطح نوکلئوتیدی مشاهده شد، هم‌چنین نتایج به دست آمده نشان داد که شناسایی علف‌های هرز میزبان که در همه‌گیری شناسی بیماری نقش مهمی دارند، در برنامه مدیریت این بیماری حائز اهمیت می‌باشد. این اولین گزارش از گیاه سلمه تره و پنیرک به عنوان میزبان علفی TYLCV در ایران می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Molecular Identification of Weed hosts of Tomato yellow leaf curl virus in southeast of Kerman Province

نویسندگان [English]

  • Kh. Salari 1
  • Atefe Hosseini 2
  • J. Heydarnejad 3
1 University of Jiroft
2 University of Birjand
3 Shahid Bahonar University of Kerman
چکیده [English]

Introduction Tomato yellow leaf curl virus, TYLCV belongs to the family Geminiviridae and Begomovirus genus (27). In recent years, extensive damage to tomatoes and cucurbits plants in the south and the southeast of Iran has arrived (23). This virus family have circular, and single-stranded DNA genome and are widespread in tropical and subtropical areas (30). They are infected several plant species with economic importance. Begomoviruses are dicot-infecting, whitefly-transmitted viruses with a genome comprised of one or two molecules DNA (5). Up to now, studies have been performed to evaluate the status of distribution, and identification of natural host and assess the genetic diversity, but there is not a comprehensive review about its weed hosts yet.
Materials and Methods In this research, The weeds from margins and inside greenhouses and farms of tomato and cucurbit in severely infected areas including Manoojan, Kahnooj, Faryab, Anbrabad and Jiroft to identify weed hosts of the virus in nature, were collected. Identification of collected samples were conducted by botanical specialists. Total DNAs were extracted from leaves according to the method of zhang et al. (1998) and stored at -20 oC. Identification of infected samples were carried out by PCR using degenerate primer pairs PCRv 181/Bc that direct the amplification of˷ 550 bp fragment of mono – and bipartite begomoviruses genome comprising the C-terminal portion of the intergenic region (IR) N-terminal portion of the CPgene. PCR were performed in 25 µl reaction volumes containing 1 µl of template DNA, o.5 µl of Taq DNA polymerase Sinaclon (IRAN), 1.2 µl MgCl2, 0.5 µl dNTPs. 1 µM of each forward and reverse primers, 4.3 µl of 10× reaction buffer and 15.5 distilled water. The amplification were performed using a peqSTAR 96x Termal Cycler (Peqlabe, Germany). PCR conditions consisted of initial denaturing 94 oC for 3 min followed by 30 cycles of denaturation at 94 oC for 50s, annealing at 55 oC for 60s, and extension at 72 oC for 1 min followed by 1 cycle at 72 oC for 10 min. Electrophoresis of polymerase chain reaction products on 1% agarose gel was performed and stained with DNA safe stain (sinaclon-IRAN). The polymerase chain reaction product were sequenced using automatic sequencer AB13730XL (Macrogen, Korea). The resulting sequences were looking similarity and after obtaining a degree of homology, 550 bp fragment of the coat protein gene of four isolates were ordinated by Bio Edit software. Looking for similar sequences were obtained and then achieved the 550 bp fragments of the coat protein gene homology four software isolates Bio edit (21) were ordination. To study the phylogenetic relationship of study strains, the phylogenetic tree was drawn with maximum likelihood way in the MEGA 5 software. Then percentage of similarity at the nucleotide and amino acid sequence with a genetic distance matrix was determined by using the software CLC Main work bench.
Results and Discussion: The results showed that four weeds including Chrozophora tinctoria, Heliotropium annum, Malva neglecta and Chenopodium murale were infected with TYLCV. To compare the TYLCV isolates in infected weeds, 550 bp fragment of the coat protein gene in four different strains of the virus was sequenced. Assessment of the genetic similarity between study isolates and strains in the Gene Bank revealed that study isolates with isolates from Gene Bank have similarity in the range of 93/24-99/98% at the nucleotide level and in the range of 87/42-98/15% at the amino acid level. Sixty-six mutations at the nucleotide level in compared sequences in this study was also found. Drawn Phylogenetic tree was confirmed the results of the genetic distance matrix. The results showed that the virus has a wide host range, and identification of weed hosts to remove the maintenance of virus plays an important role in the epidemiology of the disease. and also it’s the management of this disease. This is the first report from Malva neglecta and Chenopodium murale as weed host of TYLCV in Iran. TYLCV causing major damage to the cultivation of tomatoes. Infection caused by it in the South and South East farms in Iran is usually very widespread. Because of the subtropical climate in these areas that is essential for the activity and proliferation of whitefly vector, this virus can cause a high damage in these areas (34). High genetic variability in begomovirus populations infecting different non-cultivated hosts has been reported (28, 35, 38). Conversely, begomovirus populations infecting cultivated hosts seem to have lower variability (6,14,41,43).Wild/non-cultivated plants from different botanical families can sustain a high species diversity of begomoviruses and can play an important epidemiological role serving as alternate/reservoir hosts, preventing local extinctions of the virus when the cultivated host is absent (1, 14). In these cases, whiteflies transmitting begomoviruses between cultivated and non-cultivated hosts contribute to virus evolution and disease epidemics (1, 34). Weeds and plant host of indigenous origin and imported ones can be by a large number of plant viruses including Begomoviruses infectedwhich are as reservoirs host of the virus, the primary focus for infection of crops, and play a pivotal role in the emergence of new viral strains (35). Identification and control of the weeds have an important role in disease management. Relatively high incidence of the virus in the weeds with a high density of whitefly population throughout the year, requires new management guidelines.
Conclusion: The results showed that the virus has a wide host range, and identification of weed hosts to remove the maintenance of virus that play an important role in the epidemiology of the disease, in the management of this disease is important. This is the first report from Malva neglecta and Chenopodium murale as weed host of TYLCV in Iran.

کلیدواژه‌ها [English]

  • Begomovirus
  • Weed
  • Whitefly
  • Polymerase chain reaction
Alabi O., Ogbe F., Bandyopadhyay R., Lava Kumar P., and Dixon A. et al. 2008. Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria. Archive of Virology, 153:1743–1747.
2- Bedford I.D., Kelly A., Banks G.K., Briddon R.W., Cenis J.L., and Markham P.G. 1998. Solanum nigrum: an indigenous weed reservoir for a Tomato yellow leaf curl geminivirus in southern Spain March. European Journal of Plant Pathology, 104(2): 221-222.
3- Briddon R.W., Bull S.E., Amin I., Idris A. M., Mansoor Sh., Belford I. D., and Dhawan P. 2003. Diversity of DNA, a satellite molecular associated with some monopartite begomovirus. Virology Journal 53:763-781.
4- Brown J.K., Fauquet C.M., Briddon R.W., Zerbini M., and Moriones E. 2011. Geminiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ, eds. Virus Taxonomy - Ninth Report of the International Committee on Taxonomy of Viruses. London, Waltham, San Diego: Associated Press, Elsevier Inc. 351–373.
5- Brown J. K., Fauquet C. M., Briddon R. W., Zerbini F. M., Moriones E., and Navas-Castillo J. 2012. Family Geminiviridae. In Virus Taxonomy 9th Report of the International Committee on Taxonomy
of Viruses. 351–373.
6- Castillo-Urquiza G. P., Beserra J. E. A., Jr, Bruckner F. P., Lima A. T. M., Varsani A., Alfenas-Zerbini P., and Murilo Zerbini F. 2008. Six novel begomoviruses infecting tomato and associated weeds in southeastern Brazil. Archive of Virology 153:1985–1989.
7-Czonsek H. 2007. Tomato yellow leaf curl virus management. Molecular Biology, Breeding for resistance. Spring 2007 chapter 3: 263-278.
8- Doyle J.J., and Doyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissuse. Phytochemistry Bulletin, 19: 11-15.
9- Esmaili M., and Heydarnejad J. 2013. Identification of Wild Hosts of Watermelon Chlorotic Stunt Virus in South and Southeastern Iran. Journal of Agriculture Biotechnology, 13:2-18.
10- Fanigliulo A., Pacella R., Comes S., and Crescenzi A. 2007. Three years survey of Tomato yellow leaf curl Sardinia virus reservoir weed hosts in southern Italy. Commun Agriculture Applied Biological Science, 72(4): 1023-1028.
11-Faria J. C., and Maxwell D. P. 1999. Variability in geminivirus isolates associated with Phaseolus spp. in Brazil. Phytopathology, 89: 262–268.
12- Fauquet C.M., Briddon R.W., Brown J.K., Moriones E., Stanley J., Zerbini M., and Zhou X., 2008. Geminivirus strain demarcation and nomenclature. Archive of Virology, 153: 783-821.
13- Fazeli R, Heydarnejad J, Massumi H., and Shaabanian M. 2009. Genetic diversity and distribution of tomato-infecting begomoviruses in Iran. Virus Genes, 38: 311-319.
14- Fiallo-Olive´ E., Navas-Castillo J., Moriones E., and Martı´nez-Zubiaur Y. 2012. Begomoviruses infecting weeds in Cuba: increased host range and a novel virus infecting Sida rhombifolia. Archive of Virology, 157: 141–146.
15- Garcı´a-Andre´ S.S., Monci F., Navas-Castillo J., and Moriones E. 2006. Begomovirus genetic diversity in the native plant reservoir Solanum nigrum: evidence for the presence of a new virus species of
recombinant nature. Virology Journal, 350: 433–442.
16- Gonza´ lez-Aguilera J., Tavares S.S., Sobrinho R.R., Xavier C.A.D., Duen˜as-Hurtado F., Lara-Rodrigues R.M., Silva D.J.H., and Zerbini F.M. 2012. Genetic structure of a Brazilian population of the begomovirus Tomato severe rugose virus (ToSRV). Tropical Plant Pathology, 37: 346–353.
17- Gronenborn B. 2007. The Tomato Yellow Leaf Curl Virus genome and function of its proteins,
In: H. Czosnek, Ed., Tomato yellow leaf curl virus disease: management, molecular biology,
breeding for resistance, Springer, Dordrecht, The Netherlands, 67-84.
37- Rojas M.R., Noueiry A.O., and Lucase W.J. 1998. Bean dwarf mosaic geminivirus movment protein recognize DNA in a form-and size-specific manner. Cellular, 95: 105-113.
38- Silva SJC, Castillo-Urquiza GP, Hora-Júnior BT, Assunção IP, Lima GSA, Pio-Ribeiro G,Mizubuti ESG., and Zerbini FM . 2012. Species diversity, phylogeny and genetic variability of begomovirus populations infecting leguminous weeds in ortheastern Brazi. Plant Pathology, 61: 457-467.
39- Stanley J., Bisaro D.M., and Briddon R.W. 2005. Family Geminiviridae. In:Fauquet C.M., Mayo M.A.Virus Taxonomy, eight report of international committe on taxonomy of viruses. 301-326.
40- Tamura K., Peterson D., Peterson N., Stecher G., Nei M., and Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28: 2731-2739.
41- Tavares S. S., Ramos-Sobrinho R., Gonzalez-Aguilera J., Lima G. S. A., Assunc a˜ o, I. P., and Zerbini F. M. 2012. Further molecular characterization of weed-associated begomoviruses in Brazil with an emphasis on Sida spp. Planta Daninha, 30: 305–315.
42- Varsani A., Navas-Castillo J., Moriones E., Herna´ndez-Zepeda C.,Idris A., Brown J. K., Zerbini F. M., and Martin D. P. 2014. Establishment of three new genera in the family Geminiviridae:Becurtovirus, Eragrovirus and Turncurtovirus. Archives of Virology, 159: 2193-2203.
43- Wyant P. S., Gotthardt D., Scha fer B., Krenz B., and Jeske H. 2011. The genomes of four novel begomoviruses and a new Sida micrantha mosaic virus strain from Bolivian weeds. Archives of Virology, 156: 347–352.
44-Ying Z. T., and Davis M. J. 2000. Partial characterization and host range of Tomato yellow leaf curl virus in South Florida. Proceedings of the Florida State Horticultural Society, 113: 185-190.
45- Zhang Y.P., Uyemoto J.K., and Kirkpatrick B.C 1998. A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. Journal of Virological Methods, 71: 45-50.
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