TY - JOUR ID - 33012 TI - Serological and Molecular Detection of Tomato spotted wilt virus (TSWV) in Khorasan Razavi Province JO - Journal of Iranian Plant Protection Research JA - JPP LA - en SN - 2980-8170 AU - Shoushtari, S. AU - Jafarpour, B AU - Falahaty Rastegar, M. AD - Y1 - 2013 PY - 2013 VL - 26 IS - 4 SP - 348 EP - 354 KW - Mechanical transmission KW - RT-PCR KW - Tomato spotted wilt virus DO - 10.22067/jpp.v26i4.18418 N2 - In order to investigate the presence of Tomato spotted wilt virus ( TSWV) in tomato fields of Khorasan Razavi province ( Mashhad , Neishabour , Torbat-e-Hydarieh , Kashmar , Ghochan , Fariman , Takht-e-Golgeh and Roshtkhar ), totally 636 samples were collected from tomato fields in summer of 2008 and 2009 . The plant that showed symptoms of chlorotic , necrotic and ring spot were collected and transferred to the laboratory in cold condition , then tested by TAS-ELISA . Sap from infected plants inoculated to 3 cultivars of indicator plant e.g Nicotiana rustica, N . clevelandii and N. tabacum var Samsun . To confirme symptomology, inoculated indicator plants tested by TAS-ELISA. For molecular detection, RNA extracted by use of RNX TM(plus) kit . In RT-PCR , specific primer , amplified a fragment of 276 bp . Using RT-PCR and mechanical transmission tests , the presence of TSWV has been proven in tomato field of the province .In inoculating indicator plants only Nicotiana tabacum var Samsun (leaf deforming and mottling ) showed specific virus symptoms . UR - https://jpp.um.ac.ir/article_33012.html L1 - https://jpp.um.ac.ir/article_33012_ebc221f34adca90f0573529f675680b2.pdf ER -