بررسی منحنی کاتسکی و پارامترهای فلورسانس کلروفیل تحت تأثیر دو علف کش کلودینافوپ و Dicamba+2, 4-D

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه فردوسی مشهد

2 مرکز تحقیقات کشاورزی و منابع طبیعی خراسان رضوی

چکیده

اندازه‌گیری فلورسانس کلروفیل، روشی غیر تخریبی، سریع و بسیار حساس است که اطلاعات مهمی، در رابطه با دستگاه فتوسنتز گیاه به‌دست می‌دهد. دو آزمایش گلخانه‌ای هر کدام با پنج تکرار، جهت بررسی پارامترهای فلورسانس کلروفیل و منحنی کاتسکی در یولاف وحشی و خردل وحشی به ترتیب تحت تاثیر علف کش کلودینافوپ و Dicamba+2, 4-D انجام شد. نتایج، کاهش معنی‌داری در روند پارامترهای Fv/Fm، Fvj و Area پنج روز پس از پاشش علف کش کلودینافوپ نسبت به شاهد بدون مبارزه نشان داد در حالی که این روند یک روز پس از پاشش معنی‌دار نبود. شکل منحنی کاتسکی پنج روز پس از پاشش تحت تاثیر کلودینافوپ قرار گرفت و حداکثر فلورسانس کلروفیل (Fm) کاهش معنی‌داری پیدا کرد. پارامترهای فلورسانس کلروفیل، یک هفته زودتر از عوارض ظاهری کلودینافوپ، که به صورت زردی و خشکی برگ‌ها بروز پیدا کردند، تحت تاثیر قرار گرفت. از این خصوصیت فلورسانس کلروفیل می‌توان برای بررسی سریع و آسان‌تر کارایی علف کش‌ها نسبت به روش کلاسیک (اندازه‌گیری وزن خشک یا وزن تر) استفاده کرد. در علف هرز خردل وحشی، منحنی کاتسکی یک روز پس از پاشش علف کش Dicamba+2, 4-D در دزهای بالاتر (2/371 و 1/165 گرم ماده موثره در هکتار) دچار تغییر شد. در روز دوم پس از پاشش، کاهش فلورسانس کلروفیل، پس از زمان 1000 میلی ثانیه در دز توصیه شده (2/371 گرم ماده موثره در هکتار) اتفاق نیفتاد، اما در بقیه دزها این کاهش در هر دو روز اول و دوم پس از پاشش بوجود آمد. در نهایت اگرچه این دو علف کش بازدارنده مستقیم فتوسیستم دو نیستند اما باعث تغییر شکل منحنی کاتسکی، قبل از بروز عوارض ظاهری علف کش شدند.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of the Effects of Clodinafop and Dicamba+2, 4-D on Kautskey Curve and Chlorophyll Fluorescence

نویسندگان [English]

  • Z. Avarseji 1
  • M. H. Rashed Mohassel 1
  • A. Nezami 1
  • Majid Abbaspoor 2
  • M. Nasiri Mahallati 1
1 Ferdowsi University of Mashhad
2
چکیده [English]

Measurement of chlorophyll fluorescence is noninvasive, highly sensitive, fast and easy way to give important information about the photosynthetic apparatus. Chlorophyll fluorescence parameters and Kautsky curves of wild oat and wild mustard sprayed by clodinafop and Dicamba+2, 4-D respectively was investigated at two green house experiments. The results indicated significant reduction at trend of Fv/Fm، Fvj and Area parameters five days after clodinafop spraying. The shapes of the Kautsky curves were affected by clodinafop five days after spraying. The maximum fluorescence (Fm) reduced significantly At 5 days after spraying. Chlorophyll fluorescence parameters revealed clodinafop efficiency one week before herbicide symptoms occurred. Chlorophyll fluorescence could be an alternative method to study herbicide efficacy compare to the classical method (measuring dry or fresh weight). At wild mustard the shape of the Kautsky curve at higher doses of Dicamba+2, 4-D (371.2 and 165.1 g ai./ha) was affected one day after spray. The Kautsky curve quenching after 1000 ms did not occur two days after spray at the recommended dose (371.2 g ai./ha) but its decay was observed at under recommended doses at both one and two days after spray. Finally although clodinafop and Dicamba+2, 4-D are not directly PSII inhibitor but they could change the Kautsky curves shape and form before revealing visual symptoms of these herbicides.

کلیدواژه‌ها [English]

  • Fv/Fm
  • Fvj
  • Area
  • Dark adapted leaves
1- Abbaspoor M., and Streibig J.C. 2007. Monitoring the efficacy and metabolism of phenylcarbamates in sugar beet and black nightshade by chlorophyll fluorescence parameters. Pest Management Science, 63:576-585.
2- Abbaspoor M., and Streibig J.C. 2005. Clodinafop changes the chlorophyll fluorescence induction curve. Weed Science, 53:1-9.
3- Appenroth K.J., Stockel J., Srivastava A., and Strasser R.J. 2000. Multiple effects of chromate on the photosynthesis apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll A fluorescence measurements. Environmental Pollution, 115:49-64.
4- Barbagallo R.P., Oxborough K., Pallett K.E., and Baker N.R. 2003. Rapid, non-invasive screening for perturbations of metabolism and plant growth using chlorophyll fluorescence imaging. Plant Physiology, 132:485-493.
5- Blowers M.H. 1989. Applications of chlorophyll fluorescence to study the penetration of herbicides into leaves, University of Essex, Colchester, UK.
6- Bolhar-Nordenkampf H.R., and Oquist G. 1993. Chlorophyll fluorescence as a tool in photosynthesis research, in Photosynthesis and production in a changing environment: a field and laboratory manual, ed by Hall D.O, Scurlock J.M.O., Bolhar-Nordenkampf H.R., Leegood R.C., Long S.P., Chapman and Hall, London, pp 193–206.
7- Christensen M.G., Teicher H.B., and Streibig J.C. 2003. Linking fluorescence induction curve and biomass in herbicide screening. Pest Management Science 59:1303-1310.
8- Cobb A.H., and Reade J.P.H. 2010. Herbicides and Plant Physiology. Wiley-Blackwell; 2nd edition.United Kingdom, West Sussex.
9- Crudace A.J. 2000. The investigation of the in vivo behavior of a maize herbicide-Isoxaflutole. PhD thesis. University of Essex. Colchester, UK.
10- Daley P.F. 1995. Chlorophyll fluorescence analysis and imaging in plant stress and disease. Canadian Journal Plant Pathology -Rev Canadienne de Phytopathol 17:167–173.
11- Dat J., Vandenabeele S., Vranova E., Van Montagu M., Inze D., and Van Breusegem F. 2000. Dual action of the active oxygen species during plant stress celluar and molecular. life science, 57:779-95.
12- Dayan F.E., and Zaccaro M.L. de M. 2012. Chlorophyll fluorescence as a marker for herbicide mechanisms of action. Pesticide Biochemistry and Physiology, 102: 189–197.
13- Fayez K.A. 2000. Action of photosynthetic diuron herbicide on cell organelles and biochemical constituents of the leaves of two soybean cultivars. Pesticide Biochemistry and Physiology 66:105-115.
14- Force L., Critchey C., and Rensen J.V. 2003. New fluorescence parameters for monitoring photosynthesis in plants. Photosynthesis Research 78:17-33.
15- Fufezan C., Rutherford A.W., and Liszkaya A.K. 2002. Singlet oxygen production in herbicide-treated photosystem II. FEBS Lett. 532:407- 410.
16- Govindjee Amesz J., and Fork D.C. (Eds.), Light Emission by Plants and Bacteria, Academic Press, Orlando, 1986, 638pp.
17- Grossmann K., Kwiatkowski A., and Tresch S. 2001. Auxin herbicides induce H2O2 overproduction and tissue damage in cleavers (Galium aparine L.). Journal of experimental botany, 52: 1811-1816.
18- Habbash D., Percival M.P., and Baker N.R. 1985. Rapid chlorophyll fluorescence technique for the study of penetration of photo synthetically active herbicides into leaf tissue. Weed Research, 25:389-395.
19- Hiraki M., Rensen J.J.S.V., Vredenberg W.J., and Wakabayashi K. 2003. Characterization of the alterations of the chlorophyll a fluorescence induction curve after addition of photosystem II inhibiting herbicides. Photosynthesis Research, 78:35-46.
20- Joyard J., Teyssier E., Miege C., Berny-Seigneurin D., Marechal E., Block M.A., Dorne A.J., Rolland N., Ajlani G., and Douce R. 1998. The biochemical machinery of plastid envelope membranes, Plant Physiology, 118: 715–723.
21- Luo X.Y., Sunohara Y., and Matsumoto H. 2004. Fluazifop-butyl causes membrane peroxidation in the herbicide-susceptible broad leaf weed bristly starbur (Acanthospermum hispidum). Pesticide Biochemistry and Physiology, 78:93-102.
22- Matouskova M., Naus J., and Flasarova M. 1999. A long-term response of chlorophyll fluorescence induction to one-shot application of cyan- azine on barley plants and its relation to crop yield. Photosynthetica, 37:281-294.
23- Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance, Trends in Plant Science, 7: 405–410.
24- Newmeyer D.D., and Ferguson-Miller S. 2003. Mitochondria: releasing power for life and unleashing the machineries of death. Cell, 112: 481–490.
25- Papageorgiou G.C., Govindjee (Eds.), Chlorophyll a Fluorescence: A Signature of Photosynthesis, Advances in Photosynthesis and Respiration, vol. 19, Springer, Dordrecht, The Netherlands, 2004, 818pp.
26- Peltzer D., Dreyer E., and Polle A. 2002. Differential temperature dependencies of antioxidative enzymes in two contrasting species: Fagussylvatica and Coleus blumei. Plant Physiology and Biochemistry, 40:141-150.
27- Riethmuller-Haage I., Lammert B., Kropff M.J., Harbinson J., and Kempenaar C. 2006. Can photosynthesis-related parameters be used to establish the activity of acetolactate synthase–inhibiting herbicides on weeds? Weed Science, 54:974–982.
28- Rodriguez R. and Strasser R. 2002. The laboratory of bioenergetics. http://www.unige.ch/sciences/biologie/bioen/bioindex.html.
29- Rutherford A.W., and Krieger-Liszakay A. 2001. Herbicide-induced oxidative stress in photosystem II. Trends Biochemical Science, 26:648-653.
30- Shimabukuro R.H., Davis D.G., and Hoffer B.L. 2001. The effect of diclofop-methyl and its antagonist, vitamin E, on membrane lipids in oat (Avena sativa L.) and leafy spurge (Euphorbia esula L.). Pesticide Biochemistry and Physiology, 69:13-26.
31- Sofo A., Dichio B., Xiloyannis C., and Masia A. 2004. Effects of different irradiance levels on some antioxidant enzymes and on malondialde- hyde content during rewatering in olive tree. Plant Science, 166:293-302.
32- Stirbet A., Govindjee. 2011. On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and Photosystem II: Basics and applications of the OJIP fluorescence transient, Photochemistry and Photobiology B Biology, 104:236-257.
33- Strasser R.J., and Stirbet A.D. 2001. Estimation of the energetic connectivity of PS II centers in plants using the fluorescence rise O–J–I–P; fitting of experimental data to three different PS II models. Mathematics and Computers in Simulation, 56:451-461.
34- Theodoulou F.L., Clark I.M., He X.L., Pallett K.E., Cole D.J., and Hallahan D.L. 2003. Co-induction of glutathione-S-transferases and multidrug resistance associated protein by xenobiotics in wheat. Pest Management Science, 59:202-214.
35- Zhang J., Weaver S.E., and Hamill A.S. 2000. Risks and reliability of using herbicides at below-labeled rates. Weed Technology 14:106-115.
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